Free radical generation by selenium compounds

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5372026

Yan, L; Spallholz, J E ^[1]

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1. Texas Tech. Univ., Lubbock (United States)

Sodium selenite, sodium selenate, selenocystine (SeCys) and selenomethionine (SeMet) were tested for their ability to generate free radicals in the absence and presence of glutathione (GSH) and in the presence of cells of the human mammary tumor cell line HTB123/DU4475. Free radical generation was measured by lucigenin or luminol enhanced chemiluminescence (CL). Lucigenin CL was observed form the reaction of selenite with GSH, 2-mercaptoethanol and L-cysteine. Catalase (CT), glutathione peroxidase (GSHPx) and superoxide dismutase (SOD) suppressed CL. Heat inactivated enzymes had no suppressive inhibition of CL. Luminol CL from the reaction of selenite with GSH was much less than that observed from lucigenin CL. In the presence of the human mammary tumor cells, lucigenin CL increased 5 times the CL produced by selenite or SeCys alone and GSH in the absence of tumor cells. The enhanced CL from these reactions in the presence of tumor cells was also suppressed by CT, GSHPx and SOD. These data suggest that free radicals, mainly superoxide (O{sub 2}{sup {minus}}) anion are produced by the reaction of selenite or SeCys with GSH. In the presence of tumor cells CL was enhanced which may account for selenite and Se Cys toxicity in vitro in comparison to the lesser toxicity of selenate or SeMet.

• OSTI ID: 5372026
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOSYNTHESIS
BODY
CATALASE
DISEASES
DRUGS
ELEMENTS
ENZYMES
GLANDS
GLUTATHIONE
MAMMARY GLANDS
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDES
PEROXIDASES
POLYPEPTIDES
PROTEINS
RADICALS
RADIOPROTECTIVE SUBSTANCES
SELENIUM
SEMIMETALS
SUPEROXIDE DISMUTASE
SYNTHESIS
TOXICITY
TUMOR CELLS