Irradiation of industrial enzyme preparations. II. Characterization of fungal pectinase by thin-layer isoelectric focusing and gel filtration
Industrial dry fungal pectinase from A. niger was irradiated with doses (up to 1 Mrad) of /sup 60/Co-..gamma..rays effective in reducing microbial contamination. The pectinase was characterized by thin-layer isoelectric focusing and gel filtration in order to detect possible radiation-induced structural alterations. Thin-layer isoelectric focusing revealed at least fifteen multiple forms with pectin-depolymerizing activity, with isoelectric points in the range pH 4.5 to 7. Heterogeneity of pectinesterase was also demonstrated, the main band occurring around pH 4. By thin-layer gel filtration the molecular weight of the pectin-depolymerase was estimated as being about 36,000, and that of pectinesterase as about 33,000. Radiation-induced changes of the charge properties or molecular size of the irradiated pectinase preparation were not observed. The feasibility of using ionizing radiation for the reduction of microbial contamination of industrial enzyme preparations looks promising.
- Research Organization:
- Bundesforschungsanstalt fuer Ernahrung, Karlsruhe, Germany
- OSTI ID:
- 5370837
- Journal Information:
- J. Food Biochem.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
COBALT 60
BIOLOGICAL RADIATION EFFECTS
GAMMA RADIATION
GLYCOSYL HYDROLASES
RADIOSENSITIVITY
MEDICAL SUPPLIES
RADIOSTERILIZATION
BACTERIA
IRRADIATION
RADIOINDUCTION
ULTRASTRUCTURAL CHANGES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
COBALT ISOTOPES
ELECTROMAGNETIC RADIATION
ENZYMES
HYDROLASES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
MORPHOLOGICAL CHANGES
NUCLEI
ODD-ODD NUCLEI
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
STERILIZATION
YEARS LIVING RADIOISOTOPES
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