Rapid and sensitive radioassay for dihydroorotase
Journal Article
·
· J. Appl. Biochem.; (United States)
OSTI ID:5367780
A rapid and simple radiochemical assay for dihydroorotase (DHOase; EC 3.5.2.3) has been developed. DHOase activity is assessed by measuring the amount of L-5,6-dihydro(/sup 14/C)orotate formed from N(/sup 14/C)cerbamyl-L-aspartate in the forward reaction and N(/sup 14/C)carbamly-L-aspartate formed from L-5,6-dihydro(/sup 14/C)orotate in the reverse reaction following the separation of the two compounds by high-voltage paper electrophoresis in an acetic acid/formic acid solution, pH 2.30. The procedure can, in principle, also be applied to the assay of aspartate carbamyl transferase (EC 2.1.3.2) and dihydroorotate dehydrogenase (EC 1.3.3.1).
- Research Organization:
- Univ. of New South Wales, Kinsington
- OSTI ID:
- 5367780
- Journal Information:
- J. Appl. Biochem.; (United States), Journal Name: J. Appl. Biochem.; (United States) Vol. 1:1; ISSN JABID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON 14
CARBON ISOTOPES
DEHYDROGENASES
ENZYMES
EVEN-EVEN NUCLEI
ISOTOPES
LIGHT NUCLEI
NUCLEI
OXIDOREDUCTASES
RADIOASSAY
RADIOISOTOPES
TRANSFERASES
YEARS LIVING RADIOISOTOPES
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON 14
CARBON ISOTOPES
DEHYDROGENASES
ENZYMES
EVEN-EVEN NUCLEI
ISOTOPES
LIGHT NUCLEI
NUCLEI
OXIDOREDUCTASES
RADIOASSAY
RADIOISOTOPES
TRANSFERASES
YEARS LIVING RADIOISOTOPES