Reproductive survival and macromolecular synthesis in cultured mammalian cells exposed to single metabolites and mixtures of metabolites of benzo(a)pyrene
Chinese hamster V79 cells have been exposed to single metabolites and mixtures of metabolites of benzo(a)pyrene (B(a)P) and reproductive cell survival and certain aspects of cellular metabolism were measured. B(a)P trans-7,8-dihydrodiol plus B(a)P 1,6-quinone resulted in increased cell survival compared to treatment with either metabolite alone, the mixture thus producing a neutralizing effect. If B(a)P 4,5-oxide is mixed with 3-HOB(a)P, increasing amounts of 3-HOB(a)P do not cause any additional cell toxicity. When replicate cultures of V79 cells were exposed to either B(a)P 4,5-oxide or 6-HOB(a)P for 4, 12, or 48 h, there was an exposure-time-dependent delay in cell division, the effect being most pronounced for 48 h exposure to 6-HOB(a)P. After the division delay, cells resumed an apparently normal exponential growth rate. A larger percentage of metabolite-treated cells were cycling after treatment, than was the case with control cells, even though the exponential growth rate was similar in both sets. RNA synthesis increased above control levels after removal of the metabolite, particularly in the case of 6-HOB(a)P. Protein synthesis was inhibited by 6-HOB(a)P addition, and after metabolite removal, increased to control levels concomitantly with RNA synthesis. The results suggest that metabolite treatment causes at least a temporary cell-cycle delay. After the metabolite is removed, protein synthesis mechanisms rebound to increase protein synthesis. (ERB)
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- Sponsoring Organization:
- USDOE Office of Health and Environmental Research
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5365697
- Report Number(s):
- CONF-791059-6
- Resource Relation:
- Conference: 4. international symposium on polynuclear aromatic hydrocarbons, Columbus, OH, USA, 2 Oct 1979
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
MITOTIC DELAY
BENZOPYRENE
BIOLOGICAL EFFECTS
BIOSYNTHESIS
INHIBITION
CELL CYCLE
METABOLITES
PROTEINS
RNA
CELL CULTURES
EXPERIMENTAL DATA
GRAPHS
METABOLISM
MIXTURES
SURVIVAL TIME
TIME DEPENDENCE
TOXICITY
AROMATICS
CONDENSED AROMATICS
DATA
DATA FORMS
DISPERSIONS
HYDROCARBONS
INFORMATION
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
SYNTHESIS
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550200 - Biochemistry