High-efficiency carbohydrate fermentation to ethanol at temperatures above 40/sup 0/C by Kluyveromyces marxianus var. marxianus isolated from sugar mills
A number of yeast strains, isolated from sugar can mills and identified as strains of Kluyveromyces marxianus var. marxianus, were examined for their ability to ferment glucose and cane syrup to ethanol at high temperatures. Several strains were capable of rapid fermentation at temperatures up to 47/sup 0/C. At 43/sup 0/C, >6% (wt/vol) ethanol was produced after 12 to 14 h of fermentation, concurrent with retention of high cell viability (>80%). Although the type strain (CBS 712) of K. marxianus var. marxianus produced up to 6% (wt/vol) ethanol at 43/sup 0/C, cell viability was low, 30 to 50%, and the fermentation time was 24 to 30 h. On the basis of currently available strains, the authors suggest that it may be possible by genetic engineering to construct yeasts capable of fermenting carbohydrates at temperatures close to 50/sup 0/C to produce 10 to 15% (wt/vol) ethanol in 12 to 18 h with retention of cell viability.
- Research Organization:
- Sugar Research Institute, Mackay, Australia
- OSTI ID:
- 5365367
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 51:6; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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ALCOHOLS
ALDEHYDES
BIOCONVERSION
BIOSYNTHESIS
CARBOHYDRATES
ETHANOL
FERMENTATION
FUNGI
GLUCOSE
GRASS
HEXOSES
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
PRODUCTIVITY
SACCHARIDES
SUGAR CANE
SYNTHESIS
TEMPERATURE EFFECTS
VIABILITY
YEASTS