Sequence and nitrate regulation of the Arabidopsis thaliana mRNA encoding nitrate reductase, a metalloflavoprotein with three functional domains

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.14.5006· OSTI ID:5365126

Crawford, N M; Smith, M; Bellissimo, D; Davis, R W ^[1]

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1. Stanford Univ. School of Medicine, CA (USA)

The sequence of nitrate reductase mRNA from the plant Arabidopsis thaliana has been determined. A 3.0-kilobase-long cDNA was isolated from a {lambda}gt10 cDNA library of Arabidopsis leaf poly(A){sup +} RNA. The cDNA hybridized to a 3.2-kilobase mRNA whose level increased 15-fold in response to treatment of the plant with nitrate. An open reading frame encoding a 917 amino acid protein was found in the sequence. This protein is very similar to tobacco nitrate reductase, being >80% identical within a section of 450 amino acids. By comparing the Arabidopsis protein sequence with other protein sequences, three functional domains were deduced: (i) a molybdenum-pterin-binding domain that is similar to the molybdenum-pterin-binding domain of rat liver sulfite oxidase, (ii) a heme-binding domain that is similar to proteins in the cytochrome b{sub 5} superfamily, and (iii) an FAD-binding domain that is similar to NADH-cytochrome b{sub 5} reductase.

• DOE Contract Number: FG03-84ER13265
• OSTI ID: 5365126
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:14; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
CYTOCHROMES
DNA
DNA SEQUENCING
ENZYMES
GENE REGULATION
MESSENGER-RNA
METALLOPROTEINS
MOLECULAR STRUCTURE
NITRATES
NITROGEN COMPOUNDS
NUCLEIC ACIDS
NUCLEOTIDE DEHYDROGENASES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PIGMENTS
PROTEINS
RECOMBINANT DNA
RNA
STRUCTURAL CHEMICAL ANALYSIS