TSH stimulates 32P-labeling of thyroid nuclear HMG 14, a protein associated with actively transcribed chromatin

Journal Article · Thu Apr 01 04:00:00 EST 1982 · Endocrinology; (United States)

OSTI ID:5364500

Cooper, E; Palmer, R J; Spaulding, S W

Thyroid slices were incubated with 32P with or without TSH. 32P-labeling of acid-soluble nuclear proteins was then examined by two-dimensional polyacrylamide gel electrophoresis and autoradiography. We found that TSH enhanced the labeling of the high mobility group protein HMG 14, a protein that is preferentially associated with actively transcribed chromatin. This observation suggests that changes in HMG 14 phosphorylation may be involved in mediating TSH-induced effects on the structure and function of active chromatin.

• Research Organization: Department of Medicine, Buffalo General Hospital, State University of New York at Buffalo
• OSTI ID: 5364500
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 110:4; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMATIN
DAYS LIVING RADIOISOTOPES
DYNAMIC FUNCTION STUDIES
HORMONES
ISOTOPES
LABELLING
LIGHT NUCLEI
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
THYROID HORMONES
TSH