A Na-K-Cl cotransporter in isolated rat papillary collecting duct cells

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Kidney International; (USA)

DOI:https://doi.org/10.1038/ki.1989.180· OSTI ID:5364128

Grupp, C; Pavenstaedt-Grupp, I G; Grunewald, R W; Bevan, C; Stokes, J.B. 3d.; Kinne, R K ^[1]

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1. Max-Planck-Institut fuer Systemphysiologie, Dortmund (Germany, F.R.)

Lactate production and ion fluxes were measured in isolated rat papillary collecting duct cells (PCD) to gain further insight into the transport properties of the papillary collecting duct. Lactate production was found to be inhibited by bumetanide in a dose-dependent manner, a maximum inhibition of 22% was obtained at 10(-4) M bumetanide and an apparent Ki of 10(-8) M was determined. Bumetanide inhibition of lactate production was dependent on the presence of sodium and chloride. Chloride removal inhibited lactate production also by 20%. Bumetanide (10(-4) M) inhibited by 35% sodium uptake into PCD cells exposed to 10 mM ouabain and chloride uptake into ion depleted PCD cells by 40%. In addition, this bumetanide-sensitive chloride uptake was dependent on the presence of sodium and potassium in the incubation medium. Furthermore, 86Rb uptake into these cells was significantly reduced in the presence of 10(-4) M bumetanide. These data provide evidence for the operation of a Na-K-Cl cotransport system in rat papillary collecting duct cells. This transport system might be involved in active chloride transport in the papillary collecting duct and/or volume regulation of the PCD cells.

• OSTI ID: 5364128
• Journal Information: Kidney International; (USA), Journal Name: Kidney International; (USA) Vol. 36:2; ISSN 0085-2538; ISSN KDYIA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ALKALI METALS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHLORIDES
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
DIURETICS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELEMENTS
GLYCOSIDES
HALIDES
HALOGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LACTATES
MAMMALS
MEMBRANE TRANSPORT
METALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OUABAIN
POTASSIUM
RADIOISOTOPES
RATS
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
SODIUM COMPOUNDS
STROPHANTHINS
SYNTHESIS
TRACER TECHNIQUES
TUBULES
VERTEBRATES