Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Lability of DNA polymerase alpha correlated with decreased DNA synthesis and increased age in human cells

Journal Article · · J. Natl. Cancer Inst.; (United States)
OSTI ID:5363044
; ; ; ;
DNA excision repair and mitogen-initiated blastogenesis in human cells declined in efficiency as an apparent function of decreased DNA polymerase alpha specific activity with increased age of the cell donor. DNA polymerase alpha isolated from fetal cells contained a single, high-specific-activity enzyme form that could not be further activated and that was stable with regard to enzyme activity and affinity for DNA template-primer. DNA polymerase alpha isolated from adult-derived cells contained both low-specific-activity and high-specific-activity forms. The low-activity enzyme form, which showed low affinity of binding to DNA template-primer, was activated by treatment with phosphatidylinositol, /sup 32/P-ATP, and phosphatidylinositol kinase, resulting in a /sup 32/P-labeled enzyme that exhibited high affinity of binding to DNA template-primer. The activated enzyme was unstable, exhibiting a loss of /sup 32/P-label correlated with the loss of both specific activity and high affinity of binding to DNA template-primer. The data suggest that DNA polymerase alpha isolated from adult-derived human cells has low-activity and high-activity forms. Decreased specific activity of DNA polymerase alpha correlated with increased age of the donor appears to be a function of loss of an enzyme activator molecule resulting in diminished ability of the enzyme to bind DNA template-primer.
Research Organization:
Texas A and M Univ., College Station
OSTI ID:
5363044
Journal Information:
J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 79:6; ISSN JNCIA
Country of Publication:
United States
Language:
English

Similar Records

Structural and mechanistic investigations into a DNA polymerase from Drosophila melanogaster embryos
Thesis/Dissertation · Mon Dec 31 23:00:00 EST 1984 · OSTI ID:5253459
DNA sequence recognition protein associated with a multiprotein form of DNA polymerase alpha
Journal Article · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:5127415
HeLa cell DNA polymerase. cap alpha. is tightly associated with tryptophanyl-tRNA synthetase and diadenosine 5',5'''-P/sup 1/,P/sup 4/-tetraphosphate binding activities
Journal Article · Sat Jan 31 23:00:00 EST 1981 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:6357142

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AGE DEPENDENCE
AGED ADULTS
ANIMAL CELLS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA POLYMERASES
DNA REPLICATION
ENZYME ACTIVITY
ENZYMES
ESTERS
FIBROBLASTS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LIPIDS
LYMPHOCYTES
MATERIALS
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
POLYMERASES
RADIOISOTOPES
SOMATIC CELLS
TRACER TECHNIQUES
TRANSFERASES