Preparation and storage of isotopically labeled reduced nicotinamide adenine dinucleotide
A method for obtaining highly purified NADH in a dry, solid, and stable form is described. The method involves improvements of the ion-exchange and reversed-phase chromatographic procedures of C. J. Newton and S. M. Faynor, and D. B. Northrop. The necessary time to prepare pure NADH has been reduced to a few hours. The final product, obtained by drying the nucleotide from absolute ethanol, shows no detectable decomposition either during the drying procedure or during storage under nitrogen gas at -20 degrees C for several months. Using dry product prepared from fixed volumes of ethanolic solution, standardized solutions of known amounts of the highly purified and stored NADH can be obtained in a few seconds.
- Research Organization:
- Univ. of Wisconsin, Madison
- OSTI ID:
- 5362957
- Journal Information:
- Anal. Biochem.; (United States), Vol. 165:2
- Country of Publication:
- United States
- Language:
- English
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