Neuronal network disturbance after focal ischemia in rats

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Stroke; (USA)

DOI:https://doi.org/10.1161/01.STR.20.9.1226· OSTI ID:5359293

Kataoka, K; Hayakawa, T; Yamada, K; Mushiroi, T; Kuroda, R; Mogami, H ^[1]

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1. Osaka Univ. Medical School (Japan)

We studied functional disturbances following left middle cerebral artery occlusion in rats. Neuronal function was evaluated by (14C)2-deoxyglucose autoradiography 1 day after occlusion. We analyzed the mechanisms of change in glucose utilization outside the infarct using Fink-Heimer silver impregnation, axonal transport of wheat germ agglutinin-conjugated-horseradish peroxidase, and succinate dehydrogenase histochemistry. One day after occlusion, glucose utilization was remarkably reduced in the areas surrounding the infarct. There were many silver grains indicating degeneration of the synaptic terminals in the cortical areas surrounding the infarct and the ipsilateral cingulate cortex. Moreover, in the left thalamus where the left middle cerebral artery supplied no blood, glucose utilization significantly decreased compared with sham-operated rats. In the left thalamus, massive silver staining of degenerated synaptic terminals and decreases in succinate dehydrogenase activity were observed 4 and 5 days after occlusion. The absence of succinate dehydrogenase staining may reflect early changes in retrograde degeneration of thalamic neurons after ischemic injury of the thalamocortical pathway. Terminal degeneration even affected areas remote from the infarct: there were silver grains in the contralateral hemisphere transcallosally connected to the infarct and in the ipsilateral substantia nigra. Axonal transport study showed disruption of the corticospinal tract by subcortical ischemia; the transcallosal pathways in the cortex surrounding the infarct were preserved. The relation between neural function and the neuronal network in the area surrounding the focal cerebral infarct is discussed with regard to ischemic penumbra and diaschisis.

• OSTI ID: 5359293
• Journal Information: Stroke; (USA), Journal Name: Stroke; (USA) Vol. 20:9; ISSN SJCCA; ISSN 0039-2499
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARDIOVASCULAR DISEASES
DISEASES
ENZYME ACTIVITY
ENZYMES
GLUCOSE
HEXOSES
ISCHEMIA
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MONOSACCHARIDES
NERVES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PATHOGENESIS
PEROXIDASES
RATS
RODENTS
SACCHARIDES
VASCULAR DISEASES
VERTEBRATES