Characterization of macrophage adhesion molecule

Journal Article · Tue Jan 12 04:00:00 EST 1988 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00401a007· OSTI ID:5357864

Remold-O'Donnell, E; Savage, B

Macrophage adhesion molecule (MAM), an abundant surface molecule which functions in the adhesion and spreading of guinea pig macrophages on surfaces, is characterized as a heterodimer of the trypsin- and plasmin-sensitive glycopeptide gp160 (MAM-..cap alpha..) and the glycopeptide gp93 (MAM-..beta..). The density of MAM molecules is estimated at 630,000 per macrophage on the basis of quantitative binding of /sup 125/I-labeled monoclonal antibody. The glycopeptide subunits display microheterogeneity on isoelectrofocusing; the pI is 5.8-6.3 for gp160 (MAM-..cap alpha..) and 6.4-7.0 for gp93 (MAM-..beta..). A neutrophil gp160, gp93 molecule was shown to be indistinguishable from macrophage MAM on the basis of electrophoresis, isoelectrofocusing, and reactivity with 10 monoclonal antibodies. A related heterodimer of gp93 associated with a larger, antigenically different glycopeptide (gp180, gp93)was identified on circulating lymphocytes. Cumulative properties indicate that MAM is the guinea pig analog of human Mo1 and mouse Mac-1.

• Research Organization: Center for Blood Research, Boston, MA
• OSTI ID: 5357864
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 27:1; ISSN BICHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
FIBRINOLYSIN
FIBRINOLYTIC AGENTS
GLUCOPROTEINS
GUINEA PIGS
HEMATOLOGIC AGENTS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MEMBRANES
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PHAGOCYTES
POLYMERIZATION
PROTEINS
PURIFICATION
RADIOISOTOPES
RODENTS
SACCHARIDES
SERINE PROTEINASES
SOMATIC CELLS
TRYPSIN
TWO-DIMENSIONAL ELECTROPHORESIS
VERTEBRATES