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Effect of membrane fatty acid substitution and temperature on repair of sublethal damage in mammalian cells

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576467· OSTI ID:5355686
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Repair of sublethal radiation damage (SLD) has been investigated as a function of temperature in mouse fibroblast LM cells with different membrane lipid composition. The SLD repair after radiation was essentially the same for the cells with the more rigid (saturated fatty acid) membranes and the cells with the more fluid (polyunsaturated fatty acid) membranes. This observation was made for repair at 37/sup 0/C as well as for repair at hypothermic temperatures. Incorporation of polyunsaturated fatty acid protected the cells against hypothermic death. These experiments demonstrate that although membranes are likely targets for cell killing by low temperature treatments, membrane lipids are probably not involved in the repair of sublethal radiation damage. It must be concluded that neither the degree of polyunsaturation of the lipids nor the degree of fluidity of the membrane is important for radiation-induced killing of mammalian cells.

Research Organization:
State Univ. of Groningen, Netherlands
OSTI ID:
5355686
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 102:2; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560201 -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ESTERS
FIBROBLASTS
FLUORESCENCE
IONIZING RADIATIONS
LIPIDS
LUMINESCENCE
MAMMALS
MEMBRANES
MICE
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
TEMPERATURE EFFECTS
VERTEBRATES
X RADIATION