Active transport of. gamma. -aminobutyric acid and glycine into synaptic vesicles
- Univ. of Michigan, Ann Arbor (USA)
Although {gamma}-aminobutyric acid (GABA) and glycine are recognized as major amino acid inhibitory neurotransmitters in the central nervous system, their storage is poorly understood. In this study the authors have characterized vesicular GABA and glycine uptakes in the cerebrum and spinal cord, respectively. They present evidence that GABA and glycine are each taken up into isolated synaptic vesicles in an ATP-dependent manner and that the uptake is driven by an electrochemical proton gradient. Uptake for both amino acids exhibited kinetics with low affinity similar to a vesicular glutamate uptake. The ATP-dependent GABA uptake was not inhibited by the putative amino acid neurotransmitters glycine, taurine, glutamate, or aspartate or by GABA analogs, agonists, and antagonists. Similarly, ATP-dependent glycine uptake was hardly affected by GABA, taurine, glutamate, or aspartate or by glycine analogs or antagonists. The GABA uptake was not affected by chloride, which is in contrast to the uptake of the excitatory neurotransmitter glutamate, whereas the glycine uptake was slightly stimulated by low concentrations of chloride. Tissue distribution studies indicate that the vesicular uptake systems for GABA, glycine, and glutamate are distributed in different proportions in the cerebrum and spinal cord. These results suggest that the vesicular uptake systems for GABA, glycine, and glutamate are distinct from each other.
- OSTI ID:
- 5352997
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:10; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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AMINOBUTYRIC ACID
UPTAKE
GLYCINE
MEMBRANE TRANSPORT
BIOCHEMICAL REACTION KINETICS
TRITIUM COMPOUNDS
TISSUE DISTRIBUTION
AMINO ACIDS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOXYLIC ACIDS
DISTRIBUTION
DRUGS
HYDROGEN COMPOUNDS
KINETICS
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
REACTION KINETICS
550201* - Biochemistry- Tracer Techniques