Crystal structure of core streptavidin determined from multi-wavelength anomalous diffraction of synchrotron radiation
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
- Columbia, Univ., New York, NY (USA)
A three-dimensional crystal structure of the biotin-binding core of streptavidin has been determined at 3.1-{angstrom} resolution. The structure was analyzed from diffraction data measured at three wavelengths from a single crystal of the selenobiotinyl complex with streptavidin. Streptavidin is a tetramer with subunits arrayed in D{sub 2} symmetry. Each protomer is an 8-stranded {beta}-barrel with simple up-down topology. Biotin molecules are bound at one end of each barrel. This study demonstrates the effectiveness of multi-wavelength anomalous diffraction (MAD) procedures for macromolecular crystallography and provides a basis for detailed study of biotin-avidin interactions.
- OSTI ID:
- 5350773
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:7; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
PROTEINS
X-RAY DIFFRACTION
BIOTIN
CRYSTALLOGRAPHY
MOLECULAR STRUCTURE
SELENIUM COMPOUNDS
STEREOCHEMISTRY
STREPTOMYCES
SYNCHROTRON RADIATION
X RADIATION
AZOLES
BACTERIA
BREMSSTRAHLUNG
CARBOXYLIC ACIDS
COHERENT SCATTERING
DIFFRACTION
ELECTROMAGNETIC RADIATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
IONIZING RADIATIONS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATIONS
SCATTERING
VITAMIN B GROUP
VITAMINS
550602* - Medicine- External Radiation in Diagnostics- (1980-)
PROTEINS
X-RAY DIFFRACTION
BIOTIN
CRYSTALLOGRAPHY
MOLECULAR STRUCTURE
SELENIUM COMPOUNDS
STEREOCHEMISTRY
STREPTOMYCES
SYNCHROTRON RADIATION
X RADIATION
AZOLES
BACTERIA
BREMSSTRAHLUNG
CARBOXYLIC ACIDS
COHERENT SCATTERING
DIFFRACTION
ELECTROMAGNETIC RADIATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
IONIZING RADIATIONS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATIONS
SCATTERING
VITAMIN B GROUP
VITAMINS
550602* - Medicine- External Radiation in Diagnostics- (1980-)