Mechanisms mediating the trophic effect of nerves during vertebrate limb regeneration
Thesis/Dissertation
·
OSTI ID:5348941
Salamanders regenerate their appendages after amputation and nerves are required for this process. Experiments were designed to test the idea that one way nerves could affect blastema cell proliferation is by influencing the metabolism of extracellular matrix (ECM) components and to identify neurotrophic factors which promote blastema cell mitosis. Temporal and spatial differences of glycosaminoglycans (GAGs) synthesis is innervated and denervated limbs were examined. Hyaluronic acid (HA) was found to be the major GAG produced during the proliferative period and chondroitin sulfate during differentiation. Denervation reduced synthesis of both these components by half. Dorsal root ganglia and fibroblast growth factor (FGF), a brain-derived mitogen, similarly doubled GAG synthesis in cultured blastemas, the FGF-effect being primarily on HA production. Histochemical and autoradiographical results confirmed the biochemical data. Autoradiography of the limb tissue showed heaviest labeling of the ECM with /sup 3/H-acetate in areas which also stained most intensely with the dye, carbocyanine DBTC. Denervation reduced the staining and the radioactive labeling. These data indicate that nerves affect synthesis and accumulation of GAGs in the regenerating limb, which may be one way blastema cell proliferation is promoted.
- Research Organization:
- Indiana Univ., Bloomington (USA)
- OSTI ID:
- 5348941
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
AMINES
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
AUTORADIOGRAPHY
BIOLOGICAL FUNCTIONS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BIOSYNTHESIS
BODY
BODY AREAS
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CELL DIVISION
CELL PROLIFERATION
CHONDROITIN
FUNCTIONS
HYALURONIC ACID
ISOTOPE APPLICATIONS
LIMBS
MITOSIS
MUCOPOLYSACCHARIDES
NERVES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
POLYSACCHARIDES
RECOVERY
SACCHARIDES
SALAMANDERS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
AMINES
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
AUTORADIOGRAPHY
BIOLOGICAL FUNCTIONS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BIOSYNTHESIS
BODY
BODY AREAS
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CELL DIVISION
CELL PROLIFERATION
CHONDROITIN
FUNCTIONS
HYALURONIC ACID
ISOTOPE APPLICATIONS
LIMBS
MITOSIS
MUCOPOLYSACCHARIDES
NERVES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
POLYSACCHARIDES
RECOVERY
SACCHARIDES
SALAMANDERS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES