Enzymatic synthesis and carbon-13 nuclear magnetic resonance conformational studies of disaccharides containing. beta. -D-galactopyranosyl and. beta. -D-(1-/sup 13/C)Galactopyranosyl residues
Journal Article
·
· Biochemistry; (United States)
OSTI ID:5348148
Partially purified UDPgalactosyltransferase (EC 2.4.1.22) from bovine milk has been used to synthesize millimolar amounts of compounds such as Gal..beta..(1..-->..4)Glc, Gal..beta..(1..-->..4)GlcNAc-..beta..-hexanolamine, and Gal..beta..(1..-->..4)-GlcNAc..beta..(1..-->..4)GlcNAc. The same method has been used to prepare similar compounds containing /sup 13/C-enriched galactopyranosyl moieties. Gal..beta..(1..-->..4)GlcNAc-..beta..-hexanolamine was also synthesized in a solid-phase system in which the GlcNAc-..beta..-hexanolamine glycoside was covalently linked to agarose beads. At pH 7.0 and at 1 to 5 mM Mn/sup 2 + +/ the yields of the galactosyl saccharides are greater than 90% by using 10% excess of UDPGal donor. The use of a 90% enriched (1-/sup 13/C)galactosyl residue allowed the determination of the most abundant conformer about the galactopyranosyl-glycoside linkage by analysis of the carbon-carbon coupling constants from Cl to Gal to the C3', C4', and C5' of GlcNAc or Glc. 3 figures, 1 table.
- Research Organization:
- Michigan State Univ., East Lansing
- OSTI ID:
- 5348148
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 19:3; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Topography and polypeptide distribution of terminal N-acetylglucosamine residues on the surfaces of intact cells. Evidence for O-linked GlcNAc
Conference
·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:7108875
Synthesis and solution conformation of the type 2 blood group oligosaccharide. cap alpha. LFuc(1. -->. 2). beta. DGal(1. -->. 4). beta. DGlcNAc
Journal Article
·
Mon Mar 15 23:00:00 EST 1982
· Biochemistry; (United States)
·
OSTI ID:7071803
Topography and polypeptide distribution of terminal N-acetylglucosamine residues on the surfaces of intact cells. Evidence for O-linked GlcNAc
Thesis/Dissertation
·
Sat Dec 31 23:00:00 EST 1983
·
OSTI ID:7117845
Related Subjects
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400301* -- Organic Chemistry-- Chemical & Physicochemical Properties-- (-1987)
ALDEHYDES
ANIMALS
ATOMIC IONS
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOHYDRATES
CARBON 13
CARBON ISOTOPES
CATTLE
CHARGED PARTICLES
DISACCHARIDES
DOMESTIC ANIMALS
ENZYMES
EVEN-ODD NUCLEI
FOOD
GALACTOSE
GLUCOPROTEINS
GLYCOLIPIDS
HEXOSES
IONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MAMMALS
MANGANESE IONS
MATERIALS
MILK
MONOSACCHARIDES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PH VALUE
PROTEINS
RESONANCE
RUMINANTS
SACCHARIDES
STABLE ISOTOPES
SYNTHESIS
TRANSFERASES
VERTEBRATES
400301* -- Organic Chemistry-- Chemical & Physicochemical Properties-- (-1987)
ALDEHYDES
ANIMALS
ATOMIC IONS
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOHYDRATES
CARBON 13
CARBON ISOTOPES
CATTLE
CHARGED PARTICLES
DISACCHARIDES
DOMESTIC ANIMALS
ENZYMES
EVEN-ODD NUCLEI
FOOD
GALACTOSE
GLUCOPROTEINS
GLYCOLIPIDS
HEXOSES
IONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MAMMALS
MANGANESE IONS
MATERIALS
MILK
MONOSACCHARIDES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PH VALUE
PROTEINS
RESONANCE
RUMINANTS
SACCHARIDES
STABLE ISOTOPES
SYNTHESIS
TRANSFERASES
VERTEBRATES