Trypanosoma brucei: Enrichment by UV of intergenic transcripts from the variable surface glycoprotein gene expression site

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.9.9.4022· OSTI ID:5348109

Coquelet, H; Tebabi, P; Pays, A; Steinert, M; Pays, E ^[1]

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1. Univ. of Brussels, Rhode-Saint-Genese (Belgium)

The expression site for the variable surface glycoprotein (VSG) gene AnTat 1.3A of Trypanosoma brucei is 45 kilobases long and encompasses seven expression site-associated genes (ESAGs). After UV irradiation, several large transcripts from the putative promoter region were strongly enriched. We report that one such major transcript starts near the poly(A) addition site of the first gene (ESAG 7), spans the intergenic region, and extends to the poly(A) addition site of the second gene (ESAG 6), thus bypassing the normal 3' splice site of the ESAG 6 mRNA. Since this transcript is spliced, we conclude that UV irradiation does not inhibit splicing but stabilizes unstable processing products. This demonstrates that at least some intergenic regions of the VSG gene expression site are continuously transcribed in accordance with a polycistronic transcription model.

• OSTI ID: 5348109
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 9:9; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
DNA
ELECTROMAGNETIC RADIATION
GENE REGULATION
GENES
GLYCOPROTEINS
INVERTEBRATES
MASTIGOPHORA
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
PROTOZOA
RADIATIONS
RNA
TRANSCRIPTION
TRYPANOSOMA
ULTRAVIOLET RADIATION