Phytochrome regulation of gibberellin metabolism in shoots of dwarf Pisum sativum L

Thesis/Dissertation · Wed Jan 01 00:00:00 EST 1986

OSTI ID:5347711

Campell, B R

To study the effect of light on the recessive dwarfing allele, le, of Pisum sativum L., etiolated, paclobutrazol treated LeLe (cv. Alaska) and lele (cv. Progress) pea seedlings were transferred to different light regimes. The growth response of both genotypes to applications of GA/sub 1/, GA/sub 20/, and steviol was measured over 48 hours using position transducers. Both genotypes responded to GA/sub 1/ under red irradiation and in darkness. The LeLe plants grew in response to steviol and GA/sub 20/ under red irradiation and in darkness. The lele plants responded to steviol and GA/sub 20/ in darkness, but showed a much smaller response when red irradiated. The red effect on lele plants was reversible by far-red irradiation. (/sup 3/H)GA/sub 20/ was applied to nana pea seedlings, homozygous for le, grown under different light regimes. Radioactive metabolites were later extracted from the shoots of the treated plants. Both the free acid and conjugate pools were analyzed by reversed phase HPLC, and some radioactive metabolites were tentatively identified by comparing their retention times to those of authentic (/sup 3/H)GAs.

• Research Organization: California Univ., Davis (USA)
• OSTI ID: 5347711
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GIBBERELLIC ACID
METABOLISM
METABOLITES
RETENTION
LIQUID COLUMN CHROMATOGRAPHY
PISUM
SEEDLINGS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VISIBLE RADIATION
BACTERIA
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ELECTROMAGNETIC RADIATION
ESTERS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LACTONES
LEGUMINOSAE
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RADIATIONS
RHIZOBIUM
SEPARATION PROCESSES
550501* - Metabolism- Tracer Techniques