Chemical modification of the tryptophan residues of leucyl-tRNA synthetase by N-bromosuccinimide and 2-hydroxy-5-nitrobenzyl bromide

Journal Article · Sat Mar 01 04:00:00 EST 1986 · Sov. J. Bioorg. Chem. (Engl. Transl.); (United States)

OSTI ID:5347529

Kornelyuk, A I; Shilin, V V; Gudzera, O I; Rozhko, O T; Matsuka, G Kh

The accessibility for modification of the tryptophan residues in leucyl-tRNA synthetase from cow mammary glands has been studied with the aid of the specific chemical reagents N-bromosuccinimide and 2-hydroxy-5-nitrobenzyl bromide. UV absorption and the intrinsic fluorescence of the tryptophan residues of the enzymes were used for recording the course of the modification. It has been shown that under native conditions (pH 7.8) two superficial residues in each subunit of the dimeric enzyme undergo modification. Under denaturing conditions (6 M guanidine hydrochloride), the internal tryptophan residues are also modified. When the tryptophan residues are modified, the leucyl-tRNA synthetase is inactivated both in the aminoacylation reaction and in the ATP-PP/sub i/ exchange reaction. In the specific complex of leucyl-tRNA synthetase with tRNA/sup Leu/ one of the superficial tryptophan residues is screened and does not undergo modification by the reagents used

• Research Organization: Institute of Molecular Biology and Genetics, Kiev (USSR)
• OSTI ID: 5347529
• Journal Information: Sov. J. Bioorg. Chem. (Engl. Transl.); (United States), Journal Name: Sov. J. Bioorg. Chem. (Engl. Transl.); (United States) Vol. 11:5; ISSN SJBCD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACYLATION
ALKALI METAL COMPOUNDS
AMINO ACIDS
ANIMALS
AROMATICS
ATP
AZAARENES
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CATTLE
CHEMICAL REACTIONS
COUNTING TECHNIQUES
COWS
DAYS LIVING RADIOISOTOPES
DEACTIVATION
DICARBOXYLIC ACIDS
DOMESTIC ANIMALS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
FLUORESCENCE
GLANDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMIDES
INDOLES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LEUCINE
LIGASES
LIGHT NUCLEI
LUMINESCENCE
MAMMALS
MAMMARY GLANDS
NITRO COMPOUNDS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDATION
OXIDIZERS
OXYGEN COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PYROPHOSPHATES
PYRROLES
RADIOENZYMATIC ASSAY
RADIOISOTOPES
RNA
RUMINANTS
SCINTILLATION COUNTING
SODIUM COMPOUNDS
SPECTRA
SUCCINIC ACID
SYNTHESIS
TRYPTOPHAN
ULTRAVIOLET SPECTRA
VERTEBRATES