Inter- and intramolecular deuterium isotope effects on the cytochrome P-450-catalyzed oxidative dehalogenation of 1,1,2,2-tetrachloroethane
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
The oxidation of 1,1,2,2-tetrachloroethane to dichloroacetic acid was investigated with rat liver microsomes and purified cytochrome P-450. Deuterium substitution had no effect on Km values, but both the inter- and intramolecular isotope effects (kH/kD) on Vmax were in the range 5.7-6.1. The equivalence of the inter- and intramolecular values indicates that 6.0 may be a good estimate of the intrinsic isotope effect. The intermolecular kH/kD value for the conversion of 1,1,2,2-trichloroethane and its 1-/sup 2/H analog to chloroacetic acid was 5.5. These data, and the finding that 1 atom of /sup 18/O was incorporated into the product when TCEA was oxidized in an /sup 18/O/sub 2/ atmosphere, support an oxidative dechlorination mechanism that involves hydrogen atom abstraction by the P-450 intermediate oxo complex.
- Research Organization:
- Univ. of Colorado, Boulder
- OSTI ID:
- 5347519
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 149:2; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Technical Report
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Wed Jun 01 00:00:00 EDT 1988
·
OSTI ID:6479859
Kinetic isotope effects in cytochrome p-450-catalyzed oxidation reactions. intermolecular and intramolecular deuterium isotope effects during the n-demethylation of n,n-dimethylphentermine
Journal Article
·
Tue Jul 01 00:00:00 EDT 1980
· J. Biol. Chem.; (United States)
·
OSTI ID:5530394
Excess volumes of 1,1,2,2-tetrachloroethane or tetrachloroethene + 2-chlorotoluene, + 3-chlorotoluene, and + 4-chlorotoluene at 303.15 and 313.15 K
Journal Article
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Tue Oct 31 23:00:00 EST 1995
· Journal of Chemical and Engineering Data
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OSTI ID:137008
Related Subjects
550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
ANIMALS
BODY
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
CYTOCHROMES
DEUTERIUM
DIGESTIVE SYSTEM
ETHANE
EVEN-EVEN NUCLEI
GLANDS
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROCARBONS
HYDROGEN ISOTOPES
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPE EFFECTS
ISOTOPES
LIGHT NUCLEI
LIVER
MAMMALS
METABOLIC ACTIVATION
MICROSOMES
NUCLEI
ODD-ODD NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANOIDS
ORGANS
OXYGEN 18
OXYGEN ISOTOPES
PIGMENTS
PROTEINS
RATS
REDOX REACTIONS
RODENTS
STABLE ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
ANIMALS
BODY
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
CYTOCHROMES
DEUTERIUM
DIGESTIVE SYSTEM
ETHANE
EVEN-EVEN NUCLEI
GLANDS
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROCARBONS
HYDROGEN ISOTOPES
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPE EFFECTS
ISOTOPES
LIGHT NUCLEI
LIVER
MAMMALS
METABOLIC ACTIVATION
MICROSOMES
NUCLEI
ODD-ODD NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANOIDS
ORGANS
OXYGEN 18
OXYGEN ISOTOPES
PIGMENTS
PROTEINS
RATS
REDOX REACTIONS
RODENTS
STABLE ISOTOPES
TRACER TECHNIQUES
VERTEBRATES