Amide-conjugated indole-3-acetic acid and adventitious root formation in mung bean cuttings

Thesis/Dissertation · Wed Jan 01 04:00:00 EST 1986

OSTI ID:5347092

Norcini, J G

The purpose of this research was to investigate further the relationship between amide-conjugated auxin and adventitious root formation. Indoleacetylaspartic acid (IAA-aspartate) was positively identified as the predominant conjugate isolated from mung bean cuttings after the cuttings has been treated with 10/sup -3/ M IAA. In cuttings treated with (1-/sup 14/C)IAA immediately after excision (0 hr), the percent of extractable /sup 14/C in IAA-aspartate in the hypocotyl sharply increased until 36 hr, then steadily declined. (/sup 14/C)IAA was completely metabolized between 12 and 24 hr. The rooting activities of IAA-L-aspartate, IAA-L-alanine, and IAA-glycine were determined at various stages of root formation; some cuttings were pretreated with 10/sup -3/ M IAA at 0 hr. Pretreated cuttings that were treated with IAA-glycine at 12, 24, 36 hr exhibited the greatest consistency between replications, the greatest number of long roots, and the longest roots. The conjugates did not stimulate rooting as effectively as IAA, yet like IAA, generally enhanced rooting the greatest when applied before the first cell division (24 hr).

• Research Organization: Pennsylvania State Univ., University Park (USA)
• OSTI ID: 5347092
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETIC ACID
AMINO ACIDS
ASPARTIC ACID
AUXINS
BACTERIA
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL DIFFERENTIATION
CELL DIVISION
FOOD
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGUMINOSAE
METABOLISM
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MUNGBEANS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANT GROWTH REGULATORS
PLANTS
RHIZOBIUM
ROOTS
SEEDS
TRACER TECHNIQUES
VEGETABLES