Indomethacin augments lymphokine-activated killer cell generation by patients with malignant mesothelioma

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · Clinical Immunology and Immunopathology; (USA)

DOI:https://doi.org/10.1016/0090-1229(89)90102-5· OSTI ID:5346589

Manning, L S; Bowman, R V; Davis, M R; Musk, A W; Robinson, B W ^[1]

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1. Queen Elizabeth II Medical Centre, Nedlands (Australia)

Human malignant mesothelioma (MM) cells are resistant to natural killer (NK) cell lysis but susceptible to lysis by lymphokine-activated killer (LAK) cells from control individuals. The present study was performed to determine the capacity of patients with MM (n = 22) and individuals occupationally exposed to asbestos (the major population at risk of developing this disease, n = 52) to generate LAK cells capable of effectively lysing human mesothelioma cells. Compared to controls (n = 20), both patient groups demonstrated significantly depressed LAK cell activity against mesothelioma tumor cell targets (55 +/- 3% lysis by controls vs 34 +/- 3% lysis by patients with MM, P less than 0.005; and 45 +/- 3% lysis by asbestos-exposed individuals, P less than 0.025). Addition of 10 micrograms/ml indomethacin during LAK cell generation restored normal LAK cell activity for patients with MM (52 +/- 6% lysis of cultured human MM cells, P = NS compared to controls), suggesting that the defective cytolytic cell function observed in some patients with MM is a result of prostaglandin-induced immunosuppression. The ability of indomethacin to restore suppressed LAK cell activity in patients with MM suggests that the concomitant use of this agent in ex vivo LAK cell generation and in patients undergoing interleukin/LAK cell therapy may be beneficial.

• OSTI ID: 5346589
• Journal Information: Clinical Immunology and Immunopathology; (USA), Journal Name: Clinical Immunology and Immunopathology; (USA) Vol. 53:1; ISSN CLIIA; ISSN 0090-1229
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIPYRETICS
ASBESTOS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
ENZYME INHIBITORS
FUNCTIONS
GROWTH FACTORS
IMMUNOSUPPRESSION
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
LYSIS
MAMMALS
MAN
MATERIALS
MEMBRANES
MITOGENS
NEOPLASMS
OCCUPATIONAL DISEASES
OCCUPATIONAL EXPOSURE
ORGANIC COMPOUNDS
PATIENTS
PLEURA
PRIMATES
PROSTAGLANDINS
PROTEINS
SEROUS MEMBRANES
SOMATIC CELLS
TOXICITY
TUMOR CELLS
VERTEBRATES