Analytical scanning electron microscopy and x-ray microdiffractometry of renal calculi using etched plastic sections

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Scanning Electron Microsc.; (United States)

OSTI ID:5345700

Cheng, P T; Pritzker, K P; Tausch, J; Pittaway, A; Millard, J

We describe the technique and illustrate results of renal calculi analyses utilizing correlative analytical scanning electron microscopy and x-ray microdiffractometry on the same 100 micrometer Spurr embedded sections. Essential to the technique is the employment of an extremely sensitive x-ray microdiffractometer, and for morphological analysis, pretreatment of the sections with a sodium ethoxide etching solution. This technique facilitates more precise identification of crystalline constituents especially calcium apatite and magnesium ammonium phosphate hexahydrate (struvite). Further, the technique permits detection and mapping of both crystalline and amorphous constituents within calculi.

• Research Organization: Department of Pathology, Mount Sinai Hospital, Toronto, Canada
• OSTI ID: 5345700
• Journal Information: Scanning Electron Microsc.; (United States), Journal Name: Scanning Electron Microsc.; (United States) Vol. Pt 3; ISSN SEMYB
• Country of Publication: United States
• Language: English

Related Subjects

550602* -- Medicine-- External Radiation in Diagnostics-- (1980-)
550900 -- Pathology
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
BODY
CALCIUM COMPOUNDS
CALCULI
CARBOXYLIC ACID SALTS
CHALCOGENIDES
CHEMICAL ANALYSIS
ELECTRON MICROPROBE ANALYSIS
ELECTRON MICROSCOPY
KIDNEYS
MICROANALYSIS
MICROSCOPY
ORGANS
OXALATES
OXIDES
OXYGEN COMPOUNDS
SCANNING ELECTRON MICROSCOPY
SODIUM COMPOUNDS
SODIUM OXIDES