Uptake and localization of 3H-2 deoxy-D-glucose by retinal photoreceptors

Journal Article · Sun Jan 10 04:00:00 EST 1982 · J. Comp. Neurol.; (United States)

DOI:https://doi.org/10.1002/cne.902040202· OSTI ID:5344462

Witkovsky, P; Yang, C Y

Following dark incubation of isolated retinas of Xenopus laevis in Ringer solution supplemented with 3H-2-Deoxy-D-glucose (2DG), virtually all of the uptake of the label was by the inner segments and synaptic bases of the photoreceptor cells. Autoradiographs prepared from conventionally fixed tissue showed the same cellular distribution of label as those prepared from identically incubated, unfixed, freeze-dried retinas. However, fixation removed about 77% of the total counts. This fixation-labile, soluble fraction was identified as being primarily 2DG-6 phosphate by thin-layer chromatography. The remaining insoluble fraction corresponded in distribution to glycogen grains. In cones, glycogen is stored primarily in the paraboloid, whereas in rods it is distributed throughout the inner segment and synaptic base. EM autoradiographs illustrated that these were the sites over which fixation-resistant 2DG label was localized. Measurements of radioactivity associated with extracts of retinal glycogen following 2DG incubation demonstrated that a disproportionately high fraction of total counts were associated with the glycogen fraction. We conclude that in the amphibian retina 2DG may be incorporated into glycogen.

• Research Organization: Department of Ophthalmology, New York University Medical Center, New York
• OSTI ID: 5344462
• Journal Information: J. Comp. Neurol.; (United States), Journal Name: J. Comp. Neurol.; (United States) Vol. 204:2; ISSN JCNEA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
AUTORADIOGRAPHY
BIOLOGICAL ACCUMULATION
BODY
BODY AREAS
CARBOHYDRATES
CHROMATOGRAPHY
DISTRIBUTION
EYES
FACE
GLUCOSE
GLYCOGEN
HEAD
HEXOSES
LABELLED COMPOUNDS
METABOLISM
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
RETINA
SACCHARIDES
SENSE ORGANS
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
VERTEBRATES