Modulation of allogeneic stimulation in man. I. Characterization of an in vitro induced suppressor macrophage population
Journal Article
·
· Hum. Immunol.; (United States)
Cultured human peripheral blood mononuclear cells suppressed the allogeneic response of fresh autologous lymphocytes. This suppressor activity developed gradually over a period of one week. The cells primarily responsible for this effect were enriched by Ficoll density gradient centrifugation. It was found that the suppressor cell is a large, low density nylon wool adherent, radioresistant, phagocytic, and nonspecific esterase positive mononuclear cell. Moreover, these cells did not form E rosettes and were Fc positive. Electron microscopy confirmed that suppressor cells were macrophage like. Suppressor activity was not due to cytotoxicity, crowding, or steric hinderance by the cultured cells. The suppressor macrophage population did not appear to inhibit the allogeneic response via prostaglandin or arginase release, or interfere with the tritiated thymidine uptake by release of endogenous thymidine. The above system is viewed as an in vitro model of immune regulation by suppressor macrophages, in the context of allogeneic response.
- Research Organization:
- Sidney Farber Cancer Institute, Harvard Medical School, Boston, MA
- OSTI ID:
- 5343946
- Journal Information:
- Hum. Immunol.; (United States), Journal Name: Hum. Immunol.; (United States) Vol. 3:3; ISSN HUIMD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDASES
ANIMAL CELLS
ANIMALS
ARGINASE
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CENTRIFUGATION
CONNECTIVE TISSUE CELLS
ELECTRON MICROSCOPY
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROLASES
IMMUNOSUPPRESSION
IN VITRO
INHIBITION
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MAN
MATERIALS
MICROSCOPY
MONOCYTES
NON-PEPTIDE C-N HYDROLASES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHAGOCYTES
PHAGOCYTOSIS
PRIMATES
PROSTAGLANDINS
PYRIMIDINES
RADIOSENSITIVITY
RIBOSIDES
SEPARATION PROCESSES
SOMATIC CELLS
THYMIDINE
TIME DEPENDENCE
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMIDASES
ANIMAL CELLS
ANIMALS
ARGINASE
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CENTRIFUGATION
CONNECTIVE TISSUE CELLS
ELECTRON MICROSCOPY
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROLASES
IMMUNOSUPPRESSION
IN VITRO
INHIBITION
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MAN
MATERIALS
MICROSCOPY
MONOCYTES
NON-PEPTIDE C-N HYDROLASES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHAGOCYTES
PHAGOCYTOSIS
PRIMATES
PROSTAGLANDINS
PYRIMIDINES
RADIOSENSITIVITY
RIBOSIDES
SEPARATION PROCESSES
SOMATIC CELLS
THYMIDINE
TIME DEPENDENCE
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION
VERTEBRATES