Analysis of the subunit structure of protochlorophyllide holochrome by sodium dodecyl sulfate-polyacrylamide gel electrophoresis
The subunit structures of protochlorophyllide holochrome (PCH) and chlorophyllide holochrome (CH) were studied by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. PCH from leaves of darkgrown (Phaseolus vulgaris var. red kidney) is a polymeric pigment-protein complex of approximately 600,000 daltons. It is composed of 12 to 14 polypeptides of 45,000 daltons, when examined prior to and immediately following photoconversion. The protochlorophyllide or chlorophyllide pigment molecules are associated with these polypeptides. Subsequent to photoconversion, the absorption maximum of newly formed chlorophyllide shifts from 678 nm to 674 nm upon standing in darkness. Following the 678 to 674 spectral shift, the chlorophyllide is associated with a polypeptide with a molecular weight of 16,000 daltons. In addition, sucrose gradient centrifugation of PCH and CH under nondenaturing conditions indicates that during the course of the dark spectroscopic shift, the 600,000 dalton CH undergoes dissociation into a small chlorophyllide protein. The dissociation of CH, the change in the molecular weight of the chlorophyllide polypeptide from 45,000 to 16,000 daltons, as well as the dark spectroscopic shift are temperature-dependent and blocked below 0/sup 0/C. It was also found that each holochrome molecule of 600,000 daltons contains at least four protochlorophyllide pigment molecules.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 5340916
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 60:3; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALKALI METALS
BIOMASS
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHEMISTRY
CHLOROPHYLL
COLLOIDS
DISPERSIONS
ELECTROPHORESIS
ELEMENTS
ENERGY SOURCES
GELS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LEAVES
LEGUMINOSAE
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHASEOLUS
PHOTOCHEMISTRY
PHYTOCHROMES
PIGMENTS
PLANTS
PORPHYRINS
PROTEINS
RENEWABLE ENERGY SOURCES
SODIUM
SULFATES
SULFUR COMPOUNDS