Expression of the cloned uvr B gene of Escherichia coli: dependency on nonsense suppressors
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:5340233
Recombinant plasmid pNP5, consisting of plasmid pMB9 on which the uvrB gene is cloned, fully complements for the defects due to chromosomal uvrB mutations in the presence of the amber suppressor sup-6 or supF. Correndonuclease II activity was also completely restored in UvrB strains containing both plasmid pNP5 and amber suppressor sup-6, as compared with the parental UvrB+ strain. It is shown that the amber mutation which interferes with the expression of the cloned uvrB gene is located outside this gene. Apparently, the amber mutation exerts a polar effect on uvrB expression that is relieved by sup-6 or supF. Introduction of a rho mutation into suppressor-free UvrB strains, harboring pNP5, did not relieve the polarity caused by the amber mutation.
- Research Organization:
- Univ. of Leiden, Netherlands
- OSTI ID:
- 5340233
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 139:1; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Expression of the cloned uvrB gene of Escherichia coli: mode of transcription and orientation
Accumulation of trehalose by Escherichia coli K-12 at high osmotic pressure depends on the presence of amber suppressors
Cloning and expression in Escherichia coli cells of a plasmid pBS195 gene that determines the activity of oxygenase
Journal Article
·
Sun Jul 01 00:00:00 EDT 1979
· J. Bacteriol.; (United States)
·
OSTI ID:5334120
Accumulation of trehalose by Escherichia coli K-12 at high osmotic pressure depends on the presence of amber suppressors
Journal Article
·
Mon Aug 01 00:00:00 EDT 1988
· Journal of Bacteriology; (USA)
·
OSTI ID:6827834
Cloning and expression in Escherichia coli cells of a plasmid pBS195 gene that determines the activity of oxygenase
Journal Article
·
Tue Jan 31 23:00:00 EST 1995
· Russian Journal of Genetics
·
OSTI ID:70412
Related Subjects
550200 -- Biochemistry
550400 -- Genetics
560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
DNA
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
GENE MUTATIONS
KINETICS
MICROORGANISMS
MUTATIONS
NUCLEASES
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PHOSPHOTRANSFERASES
PLASMIDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
REACTION KINETICS
RECOVERY
REPAIR
TRANSFERASES
ULTRAVIOLET RADIATION
550400 -- Genetics
560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
DNA
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
GENE MUTATIONS
KINETICS
MICROORGANISMS
MUTATIONS
NUCLEASES
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PHOSPHOTRANSFERASES
PLASMIDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
REACTION KINETICS
RECOVERY
REPAIR
TRANSFERASES
ULTRAVIOLET RADIATION