Histone composition of nucleosomes isolated from cultured Chinese hamster cells

Journal Article · Tue Nov 01 04:00:00 EST 1977 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00641a031· OSTI ID:5335605

Rall, S C; Okinaka, R T; Strniste, G F

Nuclei isolated from cultured Chinese hamster cells were treated with micrococcal nuclease and lysed, and the resulting chromatin subunit classes (nucleosomes) were purified by sedimentation and resedimentation through isokinetic sucrose gradients. Nucleosomes isolated from (/sup 3/H)thymidine-labeled cells were analyzed for DNA size using both polyacrylamide gel and electron microscopic techniques. Nucleosomes isolated from (/sup 14/C)lysine-labeled cells were analyzed for protein content using a sodium dodecyl sulfate-polyacrylamide gel system. The results from monitoring the (/sup 14/C)lysine in each protein indicate that, in the nucleosome classes (monomer through tetramer), the molar ratios of histones H2A, H2B, H3, and H4 are equivalent. Furthermore, in each population of the nucleosome classes monomer through tetramer, it was possible to demonstrate that this histone unit (H2A + H2B + H3 + H4) is present, on the average, in the amount of two for monomers, four for dimers, six for trimers, and eight for tetramers.

• Research Organization: Los Alamos Scientific Lab., NM
• OSTI ID: 5335605
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 16:22; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
CHROMATIN
DNA
ELECTRON MICROSCOPY
ENZYMES
HAMSTERS
HISTONES
LYSINE
LYSIS
MAMMALS
MICROSCOPY
NUCLEASES
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHOTRANSFERASES
PROTEINS
RODENTS
TRANSFERASES
VERTEBRATES