Histone composition of nucleosomes isolated from cultured Chinese hamster cells

Journal Article · Tue Nov 01 00:00:00 EST 1977 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00641a031· OSTI ID:5335605

Rall, S C; Okinaka, R T; Strniste, G F

Nuclei isolated from cultured Chinese hamster cells were treated with micrococcal nuclease and lysed, and the resulting chromatin subunit classes (nucleosomes) were purified by sedimentation and resedimentation through isokinetic sucrose gradients. Nucleosomes isolated from (/sup 3/H)thymidine-labeled cells were analyzed for DNA size using both polyacrylamide gel and electron microscopic techniques. Nucleosomes isolated from (/sup 14/C)lysine-labeled cells were analyzed for protein content using a sodium dodecyl sulfate-polyacrylamide gel system. The results from monitoring the (/sup 14/C)lysine in each protein indicate that, in the nucleosome classes (monomer through tetramer), the molar ratios of histones H2A, H2B, H3, and H4 are equivalent. Furthermore, in each population of the nucleosome classes monomer through tetramer, it was possible to demonstrate that this histone unit (H2A + H2B + H3 + H4) is present, on the average, in the amount of two for monomers, four for dimers, six for trimers, and eight for tetramers.

• Research Organization: Los Alamos Scientific Lab., NM
• OSTI ID: 5335605
• Journal Information: Biochemistry; (United States), Vol. 16:22
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOCHEMISTRY
ELECTRON MICROSCOPY
CHROMATIN
DNA
HISTONES
NUCLEASES
PROTEINS
CELL CONSTITUENTS
HAMSTERS
LYSINE
LYSIS
AMINO ACIDS
ANIMALS
CARBOXYLIC ACIDS
CHEMISTRY
ENZYMES
MAMMALS
MICROSCOPY
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHOTRANSFERASES
RODENTS
TRANSFERASES
VERTEBRATES
550200* - Biochemistry