Amorphous ferrous sulfide as a reducing agent for culture of anaerobes
Amorphous ferrous sulfide, prepared by reacting ferrous ammonium sulfate and sodium sulfide, is an excellent reducing agent for the culture of anaerobes. It reduces resazurin and reacts much more rapidly with O/sub 2/ than does either soluble sulfide (HS/sup -/) or cysteine. One of the end products of the oxidation of ferrous sulfide with O/sub 2/ is red and serves as an indicator for the oxygen contamination of a culture medium. Amorphous ferrous sulfide served as a suitable reducing agent for the growth of species of Methanobacterium or Clostridium. Its use is recommended for enrichment or culture of anaerobes (e.g., autotrophs, fermentative organisms) from sediments and other habitats where organic reducing agents are undesirable and where soluble sulfide might be toxic.
- Research Organization:
- Univ. of Wisconsin, Madison
- OSTI ID:
- 5334795
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 33:2; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINO ACIDS
AMMONIUM COMPOUNDS
AMMONIUM SULFATES
ANAEROBIC CONDITIONS
BACTERIA
BIOCONVERSION
CARBOXYLIC ACIDS
CELL CULTURES
CHALCOGENIDES
CHEMICAL REACTIONS
CRYOGENIC FLUIDS
CULTURE MEDIA
CYSTEINE
ELEMENTS
FERMENTATION
FLUIDS
INDICATORS
IRON COMPOUNDS
IRON SULFATES
IRON SULFIDES
MICROORGANISMS
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN
OXYGEN COMPOUNDS
REDUCTION
SEDIMENTS
SODIUM COMPOUNDS
SODIUM SULFIDES
SOLUBILITY
SULFATES
SULFIDES
SULFUR COMPOUNDS
THIOLS
TRANSITION ELEMENT COMPOUNDS