Reaction of oxygen with radiation-induced free radicals on single-stranded polynucleotides. [40 MeV electrons]

Journal Article · Sat Oct 01 04:00:00 EDT 1977 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574553· OSTI ID:5334233

Michaels, H B; Hunt, J W

Pulse radiolysis experiments have been carried out to investigate the reaction of O/sub 2/ with the radicals produced by .OH attack on nucleic acid derivatives and single-stranded polynucleotides. The .OH adducts of the pyrimidine polynucleotides poly C and poly U react with O/sub 2/ giving rise to species whose absorption spectra are similar to the peroxy adducts of the monomers CMP and UMP. There may be two forms of polynucleotide radicals, reacting with O/sub 2/ at different rates. The situation for purine polynucleotides is complex. Radicals on single-stranded DNA react with O/sub 2/ on the same time scale as the reaction of O/sub 2/ with .OH adducts on pyrimidine polynucleotides. Typical values of the rate constant for this reaction are approximately 4 x 10/sup 8/ M/sup -1/ sec/sup -1/, a value which agrees approximately with an estimated collision frequency of O/sub 2/ with the polynucleotide radicals.

• Research Organization: Ontario Cancer Inst., Toronto
• OSTI ID: 5334233
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 72:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CRYOGENIC FLUIDS
DECOMPOSITION
DNA
ELECTRONS
ELEMENTARY PARTICLES
ELEMENTS
FERMIONS
FLUIDS
HYDROXYL RADICALS
IRRADIATION
LEPTONS
NONMETALS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
OXYGEN
PULSED IRRADIATION
RADIATION CHEMISTRY
RADIATION EFFECTS
RADICALS
RADIOINDUCTION
RADIOLYSIS