Rat nasal tissue activation of benzo(a)pyrene and 2-aminoanthracene to mutagens in Salmonella typhimurium
Journal Article
·
· Environ. Manage.; (United States)
Cytochrome P-450-dependent monooxygenase activity has been measured in the nasal turbinates of dogs and rats. The capacity of male Fischer-344 rat nasal tissue to bioactiviate benzo(a)pyrene (BaP) and 2-aminoanthracene (2-AA) to mutagens in Salmonella typhimurium was investigated. 2-AA was mutagenic in strains TA98 and TA100 when nasal tissue S-9 was utilized as the activation enzyme system and BaP was mutagenic in strain TA100. At all doses and protein concentrations tested, 2-AA displayed nearly 500-1000 times greater bacterial mutagenicity than BaP. In strain TA-100, nasal tissue S-9 was approximately twice as active toward 2-AA as lung S-9 and 75% as active as liver S-9. Aryl hydrocarbon hydroxylase activity was detected in rat nasal tissue when /sup 14/C-BaP was used as a substrate. Rat nasal tissue metabolized BaP to several oxidized metabolites which included dihydrodiols, quinones, and phenols. 3-Hydroxybenzo(a)pyrene and BaP-3,6-quinone were the major metabolites detected (150 pmoles/mg protein/30 min). These results indicate that rat nasal tissue can metabolize promutagens to reactive species which may play an important role in xenobiotic-induced nasal tumors.
- Research Organization:
- Inhalation Toxicology Research Inst., Albuquerque, NM
- OSTI ID:
- 5332523
- Journal Information:
- Environ. Manage.; (United States), Journal Name: Environ. Manage.; (United States) Vol. 5:3; ISSN EMNGD
- Country of Publication:
- United States
- Language:
- English
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560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BACTERIA
BENZOPYRENE
BODY
BODY AREAS
CARBON COMPOUNDS
CONDENSED AROMATICS
ENZYME ACTIVITY
FACE
HEAD
HYDROCARBONS
MAMMALS
METABOLIC ACTIVATION
MICROORGANISMS
MUTAGEN SCREENING
NOSE
ORGANIC COMPOUNDS
RATS
RESPIRATORY SYSTEM
RODENTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BACTERIA
BENZOPYRENE
BODY
BODY AREAS
CARBON COMPOUNDS
CONDENSED AROMATICS
ENZYME ACTIVITY
FACE
HEAD
HYDROCARBONS
MAMMALS
METABOLIC ACTIVATION
MICROORGANISMS
MUTAGEN SCREENING
NOSE
ORGANIC COMPOUNDS
RATS
RESPIRATORY SYSTEM
RODENTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
VERTEBRATES