Mechanisms of recombination and function of DNA in bacteria. Progress report, January 15, 1983-January 17, 1984

Technical Report · Sun Jan 01 04:00:00 EST 1984

OSTI ID:5327944

Guild, W R

Studies on gene transfer in pneumococcus are described. Specifically transformation by chromosomal and plasmid DNAs, transfection by phage and plasmid DNA, and the novel kind of conjugative transfer of drug resistance elements among the chromosomes of streptococci are examined. Some of these elements appear to be transposons. There are numerous questions about their structure, functions, and relation to plasmids and other better characterized genetic elements. The experiments involve extensive use of transformation, cloning in both pneumococcal and E. coli systems, and analysis of DNAs by gel electrophoresis. For restriction mapping, we have concentrated on the insertion carrying genes for resistance to chloramphenicol and tetracycline.

• Research Organization: Duke Univ., Durham, NC (USA). Dept. of Biochemistry
• DOE Contract Number: AS05-76EV03941
• OSTI ID: 5327944
• Report Number(s): DOE/EV/03941-63; ON: DE84007212
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BACTERIA
BACTERIOPHAGES
BIOLOGICAL ADAPTATION
CELL CONSTITUENTS
CHLORAMPHENICOL
CLONING
DNA
DNA SEQUENCING
DNA-CLONING
DRUGS
ELECTROPHORESIS
ENZYMES
ESCHERICHIA COLI
ESTERASES
GENETIC MAPPING
HYDROLASES
MAPPING
MICROORGANISMS
MUTANTS
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PLASMIDS
PNEUMOCOCCUS
RECOMBINANT DNA
STREPTOCOCCUS
STRUCTURAL CHEMICAL ANALYSIS
TETRACYCLINES
VIRUSES