Synthesis and evaluation of radioactive and fluorescent residualizing labels for identifying sites of plasma protein catabolism
Inulin and lactose were each coupled to tyramine by reductive amination with NaBH/sub 3/CN and the tyramine then labeled with /sup 125/I. Dilactitol-/sup 125/I-tyramine (DLT) and inulin-/sup 125/I-tyramine (InTn) were coupled by reductive amination and cyanuric chloride, respectively, to asialofetuin (ASF), fetuin and rat serum albumin (RSA). Attachment of either label had no effect on the circulating half-lives of the proteins. Radioactivity from labeled ASF was recovered in rat liver (> 90%) by 1 h post-injection and remained in liver with half-lives of 2 and 6 days, respectively, for the DLT and InTn labels. Whole body recoveries of radioactivity from DLT- and InTn labels. Whole body recoveries of radioactivity from DLT- and InTn-labeled RSA were 5 and 6.5 days, respectively, again indicating that the larger glycoconjugate label residualized more efficiently in cells following protein degradation. (Lactitol)/sub 2/-N-CH/sub 2/-CH/sub 2/-NH-fluroescein (DLF) was also coupled to ASF by reductive amination and recovered quantitatively in liver at 1 h post-injection. Native ASF was an effective competitor for clearance of DLF-ASF from the circulation. Fluorescent degradation products were retained in liver with a half-life of 1.2 days. Residualizing fluorescent labels should be useful for identification and sorting of cells active in the degradation of plasma proteins.
- Research Organization:
- Univ. of South Carolina, Columbia
- OSTI ID:
- 5326472
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
AMINATION
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOLOGICAL HALF-LIFE
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD PLASMA
BLOOD-PLASMA CLEARANCE
BODY
BODY FLUIDS
CARBOHYDRATES
CATABOLISM
CHEMICAL REACTIONS
CLEARANCE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISACCHARIDES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVALUATION
FLUORESCENCE
GLANDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
INULIN
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LACTOSE
LIVER
LUMINESCENCE
MAMMALS
MATERIALS
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SYMPATHOMIMETICS
SYNTHESIS
TRACER TECHNIQUES
TYRAMINE
VERTEBRATES