Biosynthesis and preliminary dynamics of the Y-nucleoside in yeast tRNA/sup Phe/ based on C-13 NMR experiments

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5326340

Sierzputowska-Gracz, H; Redfieldt, A; Golankiewicz, B; Agris, P F

Yeast tRNA/sup Phe/ was C-13 enriched (70 atom %) in vivo in methyl groups. Surprisingly, during the authors studies with the C-13 methyl enriched tRNA/sup Phe/, they observed a downfield carbon NMR signal at 120.9 ppm. Carbon spectra of four chemically synthesized Y-base derivatives and Q Base were compared. The authors can now unambiguously assign the downfield signal to C-13 enriched carbon number10 that is added to guanosine to form the third ring in the biosynthesis of Y base. This data encouraged the authors to continue the work on the dynamics of Y in tRNA/sup Phe/. The authors have preliminary NOE data from proton observed carbon decoupled NMR experiments at 500 MHz showing that the N-CH/sub 3/ methyl of the Y nucleoside exhibited an NOE to C1'-H of its ribose ring and to the C-2H proton of the adjacent adenosine-36 in yeast tRNA/sup Phe/. In conclusion, the presence of the C-13 enriched downfield peak in tRNA/sup Phe/ (C-13 methyl enriched) has yielded new biosynthesis information for the hypermodified nucleoside Y in tRNA. Formation of additional ring most likely starts with methylation of guanosine in position 1. Continued proton observed carbon decoupled NOE experiments will permit us to totally describe the conformation of Y in tRNA/sup Phe/ in solution.

• Research Organization: Univ. of Missouri, Columbia
• OSTI ID: 5326340
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

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550600 -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AROMATICS
AZAARENES
BARYONS
BIOCHEMISTRY
BIOSYNTHESIS
CARBON 13
CARBON ISOTOPES
CHEMISTRY
ELEMENTARY PARTICLES
EVEN-ODD NUCLEI
FERMIONS
FUNGI
GUANOSINE
HADRONS
HETEROCYCLIC COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEIC ACIDS
NUCLEONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PROTONS
PURINES
RESONANCE
RIBOSIDES
RNA
SPECTRA
STABLE ISOTOPES
SYNTHESIS
TRANSFER RNA
YEASTS