Molecular and cellular properties of human neutrophil (N) receptors for leukotriene B/sub 4/ (LTB/sub 4/)

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5325822

Goldman, D W; Gifford, L A; Marotti, T; Chernov-Rogan, T; Goetzl, E J

Human Ns express two sets of stereospecific plasma membrane receptors for the potent chemotactic factor LTB/sub 4/. Chemotaxis and increased adherence of Ns are mediated by a mean of 4400 high-affinity receptors per N with a mean dissociation constant (Kd) of 0.39 nM, whereas lysosomal enzyme release and superoxide generation are transduced by 270,000 low-affinity receptor per N with a Kd of 62 nM. The high-affinity receptors are down-regulated selectively by prior exposure of Ns to LTB/sub 4/ and require guanine nucleotide-binding proteins for optimal expression and signal transduction. Affinity-radiolabeling and solubilization of LTB/sub 4/ receptors has permitted isolation and fragmentation of the principal receptor protein in quantities sufficient for structural studies. Rabbit anti-idiotypic antibodies to a mouse IgG/sub 2b/ monoclonal anti-LTB/sub 4/ with a binding stereospecificity identical to that of N receptors for LTB/sub 4/, blocks the binding of (/sup 3/H)LTB/sub 4/ to N receptors. Anti-idiotypic antibodies to LTB/sub 4/ receptors interact perferentially with the high-affinity receptors, elicit N functional responses, and suppress selectively the stimulatory effects of LTB/sub 4/ on N chemotaxis. Thus, techniques have been developed for definitive analyses of the molecular characteristics and cellular behavior of LTB/sub 4/ receptors.

• Research Organization: Univ. of California Medical Center, San Francisco
• OSTI ID: 5325822
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
ANTIBODIES
ARACHIDONIC ACID
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MICE
MOLECULAR BIOLOGY
MONOCARBOXYLIC ACIDS
MONOCLONAL ANTIBODIES
NEUTROPHILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RABBITS
REACTION KINETICS
RECEPTORS
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES