Phorbol esters alter adenylate cyclase responses to vasoactive intestinal peptide and forskolin in the GH cell line
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5325575
Activation of protein kinase C with phorbol ester modifies cyclic AMP production in several anterior pituitary cell systems. In the GH cell line from a rat pituitary tumor, exposure to phorbol 12-myristate 13-acetate (PMA: 100 nM) for 30 minutes significantly reduces vasoactive intestinal peptide (VIP: 100 nM) stimulated adenylate cyclase (AC) activity in subsequent membrane preparations to 62 + 4% of control (n = 6 independent studies). In contrast, these same membrane preparations respond to forskolin (1 ..mu..M) with significantly more activity, 130 +/- 6% of controls (n = 6 independent studies). Finally, phorbol ester does not block an inhibitory hormone input into the AC system; somatostatin (100 nM) reduction of VIP-stimulated AC activity is not significantly different in membrane preparations from PMA treated and control cells (n = 3 independent studies). These other findings lead the authors to propose that protein kinase C can modify several sites in the AC complex in anterior pituitary cells.
- Research Organization:
- Univ. of Virginia, Charlottesville
- OSTI ID:
- 5325575
- Report Number(s):
- CONF-8606151-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
- Country of Publication:
- United States
- Language:
- English
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560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMP
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARCINOGENS
CYCLASES
DISEASES
ENDOCRINE GLANDS
ENZYME ACTIVITY
ENZYMES
ESTERS
GLANDS
LYASES
MAMMALS
NEOPLASMS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PITUITARY GLAND
PROTEINS
RATS
RODENTS
TRANSFERASES
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMP
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARCINOGENS
CYCLASES
DISEASES
ENDOCRINE GLANDS
ENZYME ACTIVITY
ENZYMES
ESTERS
GLANDS
LYASES
MAMMALS
NEOPLASMS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PITUITARY GLAND
PROTEINS
RATS
RODENTS
TRANSFERASES
VERTEBRATES