Mechanisms of ozone toxicity in cultured cells. I. Reduced clonogenic ability of polyunsaturated fatty acid-supplemented fibroblasts. Effect of vitamin E

Journal Article · Tue Dec 31 23:00:00 EST 1985 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398609530888· OSTI ID:5325330

Konings, A W

The direct action of ozone on viability and survival of normal and modified mouse lung fibroblasts has been studied. By cell manipulation of fibroblasts in culture, the content of polyunsaturated fatty acids (PUFA) in the phospholipids was increased from about 6% to about 40%. The cellular content of alpha-tocopherol (alpha-T) (vitamin E) could be drastically enhanced. Vitamin E supplementation to the cell did not influence the PUFA manipulation. Normal, PUFA, and PUFA(alpha-T) fibroblasts were exposed to ozone by bubbling 10 ppm through the cell suspensions for different periods of time (0-6 h). No significant effects of the ozone exposure could be established when normal fibroblasts were used. The PUFA fibroblasts, however, were very vulnerable to ozone toxicity, both in terms of dye uptake (Trypan blue) and cell death (clonogenic ability). When alpha-tocopherol was present in the cell (200 ng/10(6) cells), a clear protection against ozone toxicity was found. It is concluded that ozone toxicity might be higher under conditions of a relative high amount of polyunsaturated fatty acids in the membrane phospholipids of the cell and a low cellular antioxidant capacity. Cellular membranes are probably an important target for ozone-induced cell death.

• Research Organization: Univ. of Groningen, The Netherlands
• OSTI ID: 5325330
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 3; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL PATHWAYS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CLONE CELLS
CONNECTIVE TISSUE CELLS
ESTERS
FIBROBLASTS
LIPIDS
MAMMALS
MEMBRANES
MICE
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OZONE
PHOSPHOLIPIDS
RODENTS
SOMATIC CELLS
SURVIVAL TIME
TOXICITY
VERTEBRATES
VIABILITY
VITAMIN E
VITAMINS