Mitochondrial storage form of acetyl CoA carboxylase in fasted and alloxan diabetic rats

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5324592

Roman-Lopez, C R; Allred, J B

Sodium dodecyl sulfate-denatured biotinyl proteins will bind (/sup 14/C)methyl avidin which remains bound through polyacrylamide gel electrophoresis. The method has been used to demonstrate the presence of two high molecular weight subunit forms of acetyl CoA carboxylase in rat liver cytoplasm, both of which are precipitated by antibody to purifed rat liver acetyl CoA carboxylase prepared from sheep serum. Rat liver mitochondria contained five distinct biotinyl protein subunits, the two largest of which have been identified as acetyl CoA carboxylase subunits on the basis of precipitation by anti-acetyl CoA carboxylase antibody. The small quantity of acetyl CoA carboxylase associated with rat liver microsomes could be attributed to cytoplasmic contamination. The binding of radioactive avidin is sufficiently tight to use as a measure of the quantity of acetyl CoA carboxylase. The quantity and activity of the cytoplasmic enzyme was reduced in fasted and in alloxan diabetic rats compared to that in fed controls but the quantity of the enzyme associated with isolated mitochondria was not reduced. The results indicate that there is a mitochondrial storage form of acetyl CoA carboxylase.

• Research Organization: Ohio State Univ., Columbus
• OSTI ID: 5324592
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CARBON 14 COMPOUNDS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLASE
CELL CONSTITUENTS
COENZYMES
CYTOPLASM
DIABETES MELLITUS
DIGESTIVE SYSTEM
DISEASES
ELECTROPHORESIS
ENDOCRINE DISEASES
ENZYME ACTIVITY
ENZYMES
FASTING
GLANDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIVER
LYASES
MAMMALS
METABOLIC DISEASES
MICROSOMES
MITOCHONDRIA
MOLECULAR WEIGHT
ORGANOIDS
ORGANS
PATHOGENESIS
RATS
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
VERTEBRATES