Comparison of the effects of prostaglandin E/sub 2/ and parathyroid hormone on plasma calcium concentration and osteoclast function

Journal Article · Sat Sep 01 04:00:00 EDT 1979 · Endocrinology; (United States)

OSTI ID:5324573

Vanderwiel, C J; Talmage, R V

Prostaglandin Es (PGEs) are known to be stimulators of bone resorption, but there are conflicting reports as to the effects of PGE/sub 2/ and synthetic 16,16-dimethyl-PGE/sub 2/ on plasma calcium concentration. This study involves iv infusion of PGE/sub 2/ or its dimethyl analog into male thyroparathyroidectomized, parathyroidectomized, or thyroidectomized rats. Rats were given an initial dose of 5 ..mu..g PGE/sub 2/ or its analog, followed immediately by infusion at a rate of 10 ..mu..g/ml-h for up to 12 h. An additional group of rats was injected with 0.01 U parathyroid hormone. (PTH)/g BW-h. /sup 45/Ca was injected ip at either 10 h or 6 days before commencing the experiment. In contrast to PTH, PGE/sub 2/ and its analog affected bone lining cells only by increasing their quantity of filipodia. The PGs and PTH influenced osteoclast function by increasing the size of the population and the activity of individual osteoclasts. However, only PTH raised the plasma calcium level. PTH produced the expected results in plasma /sup 45/Ca and /sup 45/Ca specific activity values. PGE/sub 2/ infusion did not affect /sup 45/Ca values when /sup 45/Ca was injected 10 h before start of infusion, but it did increase /sup 45/Ca values when the isotope was injected 6 days earlier in spite of having no effect on plasma calcium concentrations. The data permit the conclusion that PGs appear to duplicate the action of PTH on osteoclasts but not on plasma calcium control. This is in support of the concept of a separation of changes in the rate of bone resorption from the control of plasma calcium concentration.

• Research Organization: Univ. of North Carolina, Chapel Hill
• OSTI ID: 5324573
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 105:3; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY
BODY FLUIDS
BONE MARROW CELLS
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
HORMONES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MEDICINE
METALS
NUCLEI
ORGANS
PARATHORMONE
PEPTIDE HORMONES
PROSTAGLANDINS
RADIOISOTOPES
RATS
RODENTS
SKELETON
SOMATIC CELLS
SURGERY
THYROIDECTOMY
TRACER TECHNIQUES
VERTEBRATES