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Meiotic and reproductive behavior of facultative apomictic BC/sub 1/ offspring derived from Pennisetum americanum-P. orientale interspecific hybrids

Journal Article · · Crop Sci.; (United States)
This study reports on the chromosome numbers, meiotic behavior, method of reproduction and fertility of BC/sub 1/ progenies from Pennisetum americanum L. Leeke, pearl millet x P. orientale L.C. Rich. interspecific hybrids backcrossed to P. americanum. Two facultatively apomictic interspecific hybrids between Pennisetum americanum, (A. chromosomes) and P. orientale (O chromosomes), 2n = 25, were pollinated with P. americanum. Sixteen backcross progenies were obtained which were of three cytotypes: 32-(14 A + 18 O), 23-(14 A + 9 O), and 27-(7 A + 20 O) chromosomes. They resulted from fertilization of unreduced gametes or partially reduce gametes by a 7 A chromosome gamete, or by development or unreduced aposporic embryo sacs, respectively. In 23 chromosome plants, the 14 A chromosomes paired mainly as bivalents or remained as univalents while the 9 O chromosomes appeared as univalents. Intergenomal pairing between P. americanum and P. orientale also were observed and could make segmental exchange possible. In the 27 chromosome progeny, the 20 O chromosomes paired, while the 7 A chromosomes remained as univalents. Meiotic behavior in 32-chromosome plants was regular with 7 A bivalents plus 9 O bivalents. The backcross progenies were male sterile but partially female fertile and produced a few seeds when pollinated with P. americanum pollen. The 23-chromosome, BC/sub 1/ progeny were reconstituted in BC/sub 2/ progenies of 32-chromosome plants x pearl millet. All BC/sub 1/ progenies had some degree of apomictic embryo sac development.
Research Organization:
USDA-ARS, Athens, GA
DOE Contract Number:
AS09-76EV00637
OSTI ID:
5324012
Journal Information:
Crop Sci.; (United States), Journal Name: Crop Sci.; (United States) Vol. 23; ISSN CRPSA
Country of Publication:
United States
Language:
English

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