Imaging of myocardial infarction in dogs and humans using monoclonal antibodies specific for human myosin heavy chains

Journal Article · Thu Aug 01 00:00:00 EDT 1991 · Journal of the American College of Cardiology; (United States)

DOI:https://doi.org/10.1016/0735-1097(91)90603-7· OSTI ID:5323919

Leger, J; Chevalier, J; Larue, C; Gautier, P; Planchenault, J; Aumaitre, E; Messner, P; Puech, P; Saccavini, J C; Pau, B ^[1]

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1. Faculte de Pharmacie, Montpellier (France)

The use of three different monoclonal antibodies specific for human ventricular myosin heavy chains in the visualization of the location and extent of necrosis in dogs with experimental acute myocardial infarction and in humans is described. Using a classic immunohistochemical method or ex vivo analysis of heart slices in dogs with acute myocardial infarction subjected to intravenous injection of unlabeled antimyosin antibodies or antimyosin antibodies labeled with indium-111, it was observed that all antibody fragments specifically reached the targeted necrotic zone less than 2 h after antibody injection and remained bound for up to 24 h. In a limited but significant number of cases (5 of the 12 humans and 11 of 43 dogs), it was possible to image the necrotic zone in vivo as early as 2 to 4 h after antibody injection. In other cases, individual blood clearance variations retarded or even prevented in vivo necrosis detection. Higher antimyosin fixation values were obtained in the necrotic zones in dogs with a rapid blood clearance relative to that of the other dogs. It is concluded that antimyosin antibodies always reached necrotic areas within 2 h. If blood clearance was rapid, in vivo imaging of the necrotic area was possible 2 to 6 h after necrosis, even in humans. In some cases, however, uncontrolled individual variations in the timing required for sufficient blood clearance hampered this rapid in vivo detection of myocardial necrosis.

• OSTI ID: 5323919
• Journal Information: Journal of the American College of Cardiology; (United States), Vol. 18:2; ISSN 0735-1097
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
HEART
SCINTISCANNING
MONOCLONAL ANTIBODIES
SPECIFICITY
MYOCARDIAL INFARCTION
DIAGNOSIS
BLOOD-PLASMA CLEARANCE
DOGS
DTPA
INDIUM 111
MAN
MYOSIN
RADIOIMMUNOSCINTIGRAPHY
AMINO ACIDS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CHELATING AGENTS
CLEARANCE
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOIMMUNODETECTION
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
TRACER TECHNIQUES
VERTEBRATES
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