Growth of plasmodium falciparum in human erythrocytes containing abnormal membrane proteins
- Albert Einstein Coll. of Medicine and Montefiore Medical Center, Bronx, NY (USA) City Univ. of New York, NY (USA)
- Albert Einstein Coll. of Medicine and Montefiore Medical Center, Bronx, NY (USA)
- St. Judes Children's Research Hospital, Memphis, TN (USA)
- Univ. of California, San Diego (USA)
To evaluate the role of erythrocyte (RBC) membrane proteins in the invasion and maturation of Plasmodium falciparum, the authors have studied, in culture, abnormal RBCs containing quantitative or qualitative membrane protein defects. These defects included hereditary spherocytosis (HS) due to decreases in the content of spectrin (HS(Sp{sup +})), hereditary elliptocytosis (HE) due to protein 4.1 deficiency (HE(4.1{sup 0})), HE due to a spectrin {alpha}I domain structural variant that results in increased content of spectrin dimers (HE(Sp{alpha}{sup I/65})), and band 3 structural variants. Parasite invasion, measured by the initial uptake of ({sup 3}H)hypoxanthine 18 hr after inoculation with merozoites, was normal in all of the pathologic RBCs. In contrast, RBCs from six HS(Sp{sup +}) subjects showed marked growth inhibition that became apparent after the first or second growth cycle. The extent of decreased parasite growth in HS(Sp{sup +}) RBCs closely correlated with the extent of RBC spectrin deficiency. Homogeneous subpopulations of dense HS RBCs exhibited decreased parasite growth to the same extent as did HS whole blood. RBCs from four HE subjects showed marked parasite growth and development.
- OSTI ID:
- 5323695
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 87:18; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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MEMBRANE PROTEINS
BIOCHEMISTRY
PLASMODIUM
GROWTH
TRITIUM COMPOUNDS
UPTAKE
ERYTHROCYTES
HEREDITARY DISEASES
HYPOXANTHINE
MALARIA
PATIENTS
ANIMALS
AROMATICS
AZAARENES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMISTRY
DISEASES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INFECTIOUS DISEASES
INVERTEBRATES
MATERIALS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PARASITIC DISEASES
PROTEINS
PROTOZOA
PURINES
SPOROZOA
550201* - Biochemistry- Tracer Techniques