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Immunolocalization of 7-2-ribonucleoprotein in the granular component of the nucleolus

Journal Article · · Experimental Cell Research; (United States)
 [1];  [2];  [3];  [4]
  1. Univ. of Erlangen-Nuernberg (West Germany)
  2. Institute of Experimental Medicine, Prague (Czechoslovakia)
  3. Univ. of Wuerzburg (West Germany)
  4. Scripps Clinic and Research Foundation, La Jolla, CA (USA)
+ Show Author Affiliations

Certain autoimmune sera contain antibodies against a nucleolar ribonucleotprotein particle associated with 7-2-RNA. In this study, the authors showed by immunofluorescence microscopy that antibodies reactive with 7-2-ribonucleoprotein immunolocalized in the granular regions of actinomycin D and 5,6-dichloro-1-{beta}-D-ribofuranosylbenzimidazole (DRB)--segregated nucleoli from Vero cells. By electron microscopic immunocytochemistry, antigen-antibody complexes were located in the granular component of transcriptionally active nucleoli from rat liver hepatocytes and HeLa cells. Anti-7-2-RNP antibodies from two autoimmune sera immunoprecipitated a major protein of M{sub r} 40,000 from ({sup 35}S) methionine-labeled HeLa cell extract. The immunolocalization data suggest that 7-2-ribonucleoprotein may be involved in stages of ribosome biogenesis which take place in the granular component of the nucleolus, i.e., assembly, maturation, and/or transport of preribosomes.

OSTI ID:
5322326
Journal Information:
Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 175:2; ISSN ECREA; ISSN 0014-4827
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMISTRY
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
EVEN-ODD NUCLEI
FLUORESCENCE
HELA CELLS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
IMMUNE SERUMS
IMMUNOASSAY
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LIVER CELLS
LUMINESCENCE
MAMMALS
METHIONINE
MICROSCOPY
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RATS
RIBOSOMES
RODENTS
SOMATIC CELLS
SUBCELLULAR DISTRIBUTION
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES