Effects of anti-C23 (nucleolin) antibody on transcription of ribosomal DNA in Chironomus salivary gland cells

Journal Article · Sat Oct 01 00:00:00 EDT 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90397-7· OSTI ID:5321997

Egyhazi, E; Pigon, A ^[1]; Chang, Jinhong; Ghaffari, S H; Dreesen, T D; Wellman, S E; Case, S T; Olson, M O.J. ^[2]

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1. Karolinska Institutet, Stockholm (Sweden)
2. Univ. of Mississippi Medical Center, Jackson (USA)

Protein C23 (also called nucleolin or 100-kDa nucleolar protein) is a major nucleolar phosphoprotein involved in ribosome biogenesis. To determine the effects of protein C23 on preribosomal RNA (pre-rRNA) synthesis anti-C23 antiserum was microinjected into nuclei of Chironomus tentans salivary glands. Transcription was measured by incubation of the glands with {sup 32}P-labeled RNA precursors followed by microdissection of nucleoli, RNA extraction, and electrophoretic analyses. Injection of the anti-C23 antibody caused a 2- to 3.5-fold stimulation of {sup 32}P incorporation into 38 S pre-rRNA. No stimulation was observed in salivary glands injected with preimmune serum or antiserum preabsorbed with protein C23. The stimulatory effect was selective for pre-rRNA as indicated by the lack of stimulation of {sup 32}P incorporation into extranucleolar RNA. Injection of the antiserum produced little or no effect on pre-RNA processing as measured by the relative amounts of {sup 32}P-labeled intermediate cleavage products of pre-rRNA in stimulated versus control glands. These results suggest that protein C23 not only is involved in ribosome assembly but also plays a role in regulating the transcription of the preribosomal RNA.

• OSTI ID: 5321997
• Journal Information: Experimental Cell Research; (United States), Vol. 178:2; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NUCLEOPROTEINS
BIOCHEMISTRY
RIBOSOMAL RNA
BIOSYNTHESIS
ANTIBODIES
INSECTS
PHOSPHORUS 32
RIBOSOMES
SALIVARY GLANDS
TRANSCRIPTION
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
GLANDS
INVERTEBRATES
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
SYNTHESIS
550401* - Genetics- Tracer Techniques