Urinary trimethylselenonium ion (TMSe) as an index of selenium (Se) exposure in rats
Large doses of certain Se compounds have been suggested as cancer chemoprevention agents in humans and better techniques for assessing Se status at such intakes are needed. Others have shown that urinary TMSe excretion is a function of Se exposure in rats and an HPLC method has recently been developed for measuring TMSe in urine. In their studies, young male rats were injected i.p. with 6 to 600 ..mu..g Se as selenite or L-selenomethionine containing 3..mu..Ci /sup 75/Se as the appropriate tracer. Urine was collected for 24 hours and desalted. TMSe was separated by HPLC on a strong cation exchanger with NH/sub 4/C/sub 2/H/sub 3/O/sub 2/ (pH 5.0) as the eluting buffer. Identification of TMSe was confirmed by autoradiographic TLC vs. authentic standards. The fraction of urinary Se excreted as TMSe increased from 2 to 70% as the amount of selenite injected increased. Only 3 to 53% appeared in urine as TMSe after injection of equivalent doses of selenomethionine. There was little or no effect of crude (chow) vs. purified (casein) diet on the excretion of TMSe after selenite injection, but Se-deficient rats excreted more Se in the form of TMSe than Se-supplemented rats. Urinary TMSe is a promising tool for assessing Se exposure but more work is needed to evaluate the effect of various dietary forms of Se on the excretion of TMSe in the urine.
- Research Organization:
- Dept. of Agriculture, Beltsville, MD
- OSTI ID:
- 5320822
- Report Number(s):
- CONF-8604222-; TRN: 86-028431
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Urinary trimethylselenonium excretion by the rat: effect of level and source of selenium-75
Selenosugar and trimethylselenonium among urinary Se metabolites: dose- and age-related changes
Related Subjects
SELENIUM
METABOLISM
AUTORADIOGRAPHY
EXCRETION
LIQUID COLUMN CHROMATOGRAPHY
RATS
SELENITES
SELENIUM 75
URINE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHROMATOGRAPHY
CLEARANCE
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MATERIALS
NUCLEI
OXYGEN COMPOUNDS
RADIOISOTOPES
RODENTS
SELENIUM COMPOUNDS
SELENIUM ISOTOPES
SEMIMETALS
SEPARATION PROCESSES
VERTEBRATES
WASTES
550501* - Metabolism- Tracer Techniques