Urinary trimethylselenonium ion (TMSe) as an index of selenium (Se) exposure in rats

Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5320822

Morris, V C; Levander, O A

Large doses of certain Se compounds have been suggested as cancer chemoprevention agents in humans and better techniques for assessing Se status at such intakes are needed. Others have shown that urinary TMSe excretion is a function of Se exposure in rats and an HPLC method has recently been developed for measuring TMSe in urine. In their studies, young male rats were injected i.p. with 6 to 600 ..mu..g Se as selenite or L-selenomethionine containing 3..mu..Ci /sup 75/Se as the appropriate tracer. Urine was collected for 24 hours and desalted. TMSe was separated by HPLC on a strong cation exchanger with NH/sub 4/C/sub 2/H/sub 3/O/sub 2/ (pH 5.0) as the eluting buffer. Identification of TMSe was confirmed by autoradiographic TLC vs. authentic standards. The fraction of urinary Se excreted as TMSe increased from 2 to 70% as the amount of selenite injected increased. Only 3 to 53% appeared in urine as TMSe after injection of equivalent doses of selenomethionine. There was little or no effect of crude (chow) vs. purified (casein) diet on the excretion of TMSe after selenite injection, but Se-deficient rats excreted more Se in the form of TMSe than Se-supplemented rats. Urinary TMSe is a promising tool for assessing Se exposure but more work is needed to evaluate the effect of various dietary forms of Se on the excretion of TMSe in the urine.

• Research Organization: Dept. of Agriculture, Beltsville, MD
• OSTI ID: 5320822
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHROMATOGRAPHY
CLEARANCE
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
EXCRETION
INTERMEDIATE MASS NUCLEI
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MATERIALS
METABOLISM
NUCLEI
OXYGEN COMPOUNDS
RADIOISOTOPES
RATS
RODENTS
SELENITES
SELENIUM
SELENIUM 75
SELENIUM COMPOUNDS
SELENIUM ISOTOPES
SEMIMETALS
SEPARATION PROCESSES
URINE
VERTEBRATES
WASTES