Optimization and application of thermospray high-performance liquid chromatography/mass spectrometry

Journal Article · Wed May 01 04:00:00 EDT 1985 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00283a007· OSTI ID:5320680

Voyksner, R D; Haney, C A

Thermospray (TSP) HPLC/MS frequently produces molecular weight information and exhibits lower detection limits than other HPLC/MS techniques for certain compounds. Thermospray exhibits the best sensitivities when used with a 0.1 M ammonium acetate buffer in a high percent water solvent system. The optimal interface temperatures vary with solvent composition. The optimal temperatures can be determined by maximizing the solvent-buffer ion intensities. Thermospray ionization resembles ammonia CI, which produces protonation, ammonium addition, and proton-bound solvent molecule clusters depending on the basicity of the analyte and existing thermodynamics in the source. The ionization process is soft; very few fragment ions are observed. The use of TSP HPLC/MS for the analysis of triazine herbicides and organophosphorus pesticides shows excellent specificity and sensitivity. Thermospray spectra of these compounds were quite simple, consisting of an (M + H)/sup +/ or (M + NH/sub 4/)/sup +/ ion with no fragment ion detected. Detection limits for these compounds varied from 10 to 300 ng and full scan spectra were obtained (S/N of about 5 for the base peak). 36 references, 12 figures, 2 tables.

• Research Organization: Research Triangle Institute, Research Triangle Park, NC
• OSTI ID: 5320680
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 57:6; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
400104* -- Spectral Procedures-- (-1987)
ACETATES
AMMONIUM COMPOUNDS
AQUEOUS SOLUTIONS
AZINES
BUFFERS
CARBOXYLIC ACID SALTS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
DISPERSIONS
EXPERIMENTAL DATA
HERBICIDES
HETEROCYCLIC COMPOUNDS
INFORMATION
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
MIXTURES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOMETALLIC COMPOUNDS
PESTICIDES
PH VALUE
QUANTITATIVE CHEMICAL ANALYSIS
SENSITIVITY
SEPARATION PROCESSES
SOLUTIONS
SPECIFICITY
SPECTROSCOPY
TEMPERATURE DEPENDENCE
THERMODYNAMICS
TRACE AMOUNTS
TRIAZINES