Metabolic activation of nitropyrenes and diesel-particulate extracts. Research report Aug 83-Jul 88

Technical Report · Mon Jan 01 04:00:00 EST 1990

OSTI ID:5320449

Jeffrey, A M; Santella, R M; Wong, D; Hsieh, L L; Heisig, V

The study compared DNA adducts formed between 1-nitropyrene (1-NP), 1,3-dinitropyrene(1,3-DNP), 1,6-dinitropyrene(1,6-DNP), or 1,8-dinitropyrene(1,8-DNP) and cellular DNA in various systems (including human bronchial segments, rabbit lung and trachea, and mouse embryo fibroblast C3H/10T1/2 cells) with those formed in Salmonella typhimurium. The study administered radiolabeled nitropyrenes, and isolated and digested the modified DNA. By HPLC, elution times of the radioactive adducts were compared with synthetic standards. Metabolism of 1-NP was most evident in the human bronchial tissue. Little metabolism occurred in duodenal and colonic samples. Mouse C3H/10T1/2 embryo fibroblasts showed no detectable metabolism of, DNA adduct formation with, or transformation by 1-NP. DNA adducts occurred in cells exposed to 1,8-DNP and traces of adducts formed with either 1,3-DNP or 1,6-DNP. DNA adducts after treatment with (4,5,9,10-(3)H)-1-NP in rabbit tracheal samples showed that 2 to 15 percent could be accounted for as the C-8 adduct. The study used antibodies to detect 1-NP adducts in S. typhimurium TA 1538. They detected adducts in the livers of female, but not male, rats treated with 1-NP. The study found that these antisera showed cross-reactivity with other DNA adducts. The study used (32)P-postlabeling to analyze DNA samples from animals exposed for 30 months to high levels of diesel engine emissions. This exposure resulted in elevated levels of DNA adducts.

• Research Organization: Columbia Univ., New York, NY (United States). Coll. of Physicians and Surgeons
• OSTI ID: 5320449
• Report Number(s): PB-91-172023/XAB
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BODY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIESEL ENGINES
DIGESTIVE SYSTEM
DNA ADDUCTS
ENGINES
EXHAUST GASES
FLUIDS
GASEOUS WASTES
GASES
GLANDS
HEAT ENGINES
IMMUNOASSAY
INTERNAL COMBUSTION ENGINES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MAMMALS
MAN
METABOLIC ACTIVATION
MICE
NITRO COMPOUNDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARTICLES
PARTICULATES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYCYCLIC NITRO COMPOUNDS
PRIMATES
RABBITS
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
SEX DEPENDENCE
SPECIES DIVERSITY
TRACER TECHNIQUES
VERTEBRATES
WASTES