Influence of long-term low levels of ozone on the leaf surface mycoflora of pinto bean plants
Pinto bean plants (Phaseols vulgaris strain 111) were grown for 4, 7, 14, 21, or 28 days in greenhouses containing ambient air charcoal-filtered air or ozone at 6 pphm for 8 hr/day. Ozone was produced with a Welbach generator and monitored with Mast meters. The upper and lower surfaces of the first set of simple true leaves were used to make leaf prints on acidified potato-dextrose agar plates (PDA) at each sampling period. Discs cut from these leaves were washed 10 times in sterile water and plated on PDA. Results with leaf prints showed that species of 25 genera of fungi were present in recognizable successions on all leaves. The number of fungi per cm/sup 2/ leaf tissue increased with leaf age for all leaves, with the greatest number occurring on 28-day-old leaves with accumulated ozone flecks. Differences between leaves by sources was more quantitative than qualitative, with the exception of Aspergillus niger, which was common only on the leaves of plants grown in ambient air. Botrytis cincrea was commonly found on plates printed with leaves that had ozone fleck. Isolates of Candida, Cryptococcus, and Penicillium were the most abundant fungi on all leaves. Similar results were obtained with plated washed leaf discs except that the number of fungi genera present was reduced from 25 to 11.
- Research Organization:
- Univ. of Massachusetts, Waltham
- OSTI ID:
- 5319529
- Journal Information:
- Phytopathology; (United States), Vol. 62:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FUNGI
INFECTIVITY
OZONE
TOXICITY
PHASEOLUS
PLANT DISEASES
AGE DEPENDENCE
AIR POLLUTION
BIOLOGICAL VARIABILITY
CONTROLLED ATMOSPHERES
LEAVES
ATMOSPHERES
LEGUMINOSAE
PLANTS
POLLUTION
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)